ARG81298
ALT / Alanine Transaminase Assay Kit
ALT / Alanine Transaminase Assay Kit for Functional study and Human,Mouse,Rat,Mammal
Overview
| Product Description | ARG81298 ALT / Alanine Transaminase Assay Kit is a detection kit for the quantification of ALT / Alanine Transaminase in serum and plasma. |
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| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | FuncSt |
| Specificity | Alanine Transaminase (ALT) facilitates the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. Then pyruvate and NADH are converted to lactate and NAD by lactate dehydrogenase (LDH). Therefore, the decrease in NADH absorbance at 340 nm is proportionate to ALT activity. |
| Target Name | ALT / Alanine Transaminase |
| Conjugation Note | Read at 340 nm. |
| Sensitivity | 2 U/l |
| Detection Range | 2 - 100 U/l |
| Sample Type | Serum and plasma. |
| Sample Volume | 20 µl |
| Alternate Names | Glutamate pyruvate transaminase 1; AAT1; GPT1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Alanine aminotransferase 1; ALT1; EC 2.6.1.2 |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 10 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GPT |
| Gene Full Name | glutamic-pyruvate transaminase (alanine aminotransferase) |
| Background | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| Function | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81298 ALT / Alanine Transaminase Assay Kit functional study image
Functional study: ARG81298 ALT / Alanine Transaminase Assay Kit results of a typical example of ALT activity reading at 340 nm.
| Title | Download Link |
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| ARG81298 ALT Assay Kit User's manual |
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