ARG82127
ACHE Activity Assay Kit (Colorimetric)
ACHE Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species
Overview
| Product Description | ARG82127 ACHE Activity Assay Kit (Colorimetric) can be used to measure ACHE activity in serum, plasma and blood. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | ACHE |
| Conjugation Note | Read at 412 nm |
| Sensitivity | 10 U/L |
| Detection Range | 10 - 600 U/L |
| Sample Type | serum, plasma and blood |
| Sample Volume | 10 µl |
| Alternate Names | ARACHE; Acetylcholinesterase; ACEE; EC 3.1.1.7; AChE; N-ACHE; YT |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
|---|---|
| Assay Time | 10 min |
Properties
| Form | Liquid |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACHE |
| Gene Full Name | acetylcholinesterase (Yt blood group) |
| Background | Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008] |
| Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. [UniProt] |
| Cellular Localization | Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein. Isoform T: Nucleus. Note=Only observed in apoptotic nuclei. Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82127 Acetylcholinesterase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 or 1000 U/L Acetylcholinesterase reaction, using ARG82127 Acetylcholinesterase Activity Assay Kit (Colorimetric).
| Title | Download Link |
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| ARG82127 Acetylcholinesterase Activity Assay Kit (Colorimetric) User's manual |
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