Bioactive proteins for cell-based functional assays
Bioactive proteins for cell-based functional assays
Recombinant proteins are widely used in biological research. Non-bioactive proteins are restricted in application of immunoassays such as WB and ELISA. Only biologically active proteins are useful for stimulating biological process.
arigo offers a series of bioactive recombinant proteins which are validated in mammalian cells for proliferation, cytokine secretion, chemotaxis, cytotoxicity, and more cellular responses. The bioactivity are quantified as ED50. arigo’s active proteins are ideal to investigate signaling pathways, cell differentiation, and more functional cell-based assays.
Benefits
- Validated in mammalian cells
- Activity expressed as ED50 (effective dose)
- Low endotoxin levels
- High purity and lot-to-lot consistency
Applied for
arigo’s Active Protein list
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● M1/M2 polarization and primary macrophage culture
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Macrophages are classified into inflammatory M1 or resolving M2 phenotypes. In laboratories, M1 and M2 macrophage polarization can be induced by cytokines IFN-g and IL4/IL-13 respectively. In addition, cytokines GM-CSF and M-CSF are required for primary macrophages culture. All the above proteins as well as validation solutions for M1/M2 polarization are available in arigo. |
| Human | Mouse | |
| IFN-γ | ARG70035 | ARG70372 |
| IL-4 | ARG70047 | ARG70156 |
| IL-13 | ARG70057 | ARG70165 |
| GM-CSF | ARG70037 | ARG70192 |
| M-CSF | ARG70133 | ARG70193 |
● Cell fate transition
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TGF-β, plays a crucial role in embryonic development, is widely used as an inducer for triggering cell fate transitions such as EMT, EndMT, and myofibroblast transdifferentiation. Besides, TGF-β is also frequently used for studying the signaling transduction including Smad2/3 and MAPK pathways. arigo offers quality TGF-β proteins and a serious validation tools to facilitate the research of cell fate transition and signaling. |
Active protein |
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Cell fate transition |
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Signaling |
Smad2 pSer467 antibody (ARG55037) |
● Cell culture supplements
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Growth factors are required for cell proliferation and development. They are important supplements for cell culture. For example, bFGF is critical to maintain stem cell culture in an undifferentiated state. BDNF and GDNF are used to differentiate human pluripotent stem cell-derived neural progenitor cells into neurons. arigo offers pure and high-quality active growth factor proteins that are ideal cell culture supplements. |
| Human | Mouse | |
| bFGF | ARG70109 | ARG70189 |
| EGF | ARG70136 | ARG70195 |
| BDNF | ARG70141 | |
| GDNF | ARG70143 | |
| CTNF | ARG70142 | ARG70199 |
| bNGF | ARG70145 | ARG70200 |
| VEGF121 | ARG70137 | ARG70196 |
| VEGF165 | ARG70138 | ARG70197 |
