ARG70192
Mouse GM-CSF recombinant protein (Active) (His-tagged, N-ter)
Mouse GM-CSF recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
Product Description | E. coli expressed, His-tagged (N-ter) Active Mouse GM-CSF recombinant protein |
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Tested Application | SDS-PAGE |
Target Name | GM-CSF |
Species | Mouse |
A.A. Sequence | Ala18 - Lys141 |
Expression System | E. coli |
Activity | Active |
Activity Note | Determined by its ability to induce proliferation in FDC-P1 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2x 10^7 IU/mg. |
Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Properties
Form | Powder |
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Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
Purity | >98% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) |
Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Gene Symbol | CSF2 |
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Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
Cellular Localization | Secreted. [UniProt] |
Highlight | Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
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