ARG30322
Myofibroblast Differentiation Antibody Duo
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Publication28
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG66199 | anti-Vimentin antibody [SQab1721] | Rabbit mAb | Hu, Ms | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB | 50 μl |
| ARG66381 | anti-alpha smooth muscle Actin antibody [SQab18108] | Rabbit mAb | Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh | FACS, ICC/IF, IHC-Fr, IHC-P, WB | 50 μl |
Overview
| Product Description | Myofibroblast differentiation is a highly regulated process and plays a critical role in tissue repair, wound healing and chronic fibrosis. This antibody duo comprises fibroblast marker Vimentin antibody and myofibroblast marker smooth muscle actin antibody. It is ideal for the research of fibroblast-to-myofibroblast differentiation. Related news: New antibody panels for Myofibroblasts and CAFs New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
|---|---|
| Target Name | Myofibroblast Differentiation |
| Alternate Names | CAF Marker antibody; Cancer-associated fibroblast Marker antibody; Vimentin antibody; alpha smooth muscle Actin antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Cancer-associated fibroblast (CAF) Marker Antibody Duo |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
Images (14) Click the Picture to Zoom In
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ARG66199 anti-Vimentin antibody [SQab1721] WB image
Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image
Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.
From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.
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ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image
Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.
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ARG66199 anti-Vimentin antibody [SQab1721] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.
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ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
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ARG66199 anti-Vimentin antibody [SQab1721] IP image
Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).
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ARG66199 anti-Vimentin antibody [SQab1721] WB image
Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
Specific References
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
ARG66381: IHC-P / Mouse
Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction
ARG66381: WB / Mouse
Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs
ARG66381: IHC-P / Bovine
Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice
ARG66381: IHC-P / Mouse
Comparison of the biological effects of various radiation therapy for keloid by single-cell RNA sequencing reveals IRF1 as a novel target in keloid therapy
ARG66199: ICC/IF / Human
TRIM28 promotes tumor growth and metastasis in breast cancer by targeting the BRD7 protein for ubiquitination and degradation
ARG66199: IHC-P / Human
PCSK9 inhibitors ameliorate arterial stiffness in ACS patients: evidences from mendelian randomization, cohort studies and basic experiments
ARG66381: WB / Mouse
Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels
Cattle / ARG66381: IHC-P
CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization
Mouse / ARG66381: IHC-P
BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma
ARG66199: WB / Human
Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis
ARG66381: IHC-P / Mouse
Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet
ARG66381: IFC-Fr / Mouse
Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression
ARG66199: WB / Human
Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h
ARG66199: WB / Human
Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells
ARG66199: WB, IHC-P / Human
Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma
ARG66381: IHC-P / Human
Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes
ARG66199: IHC-Fr / Mouse
Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma
ARG66381: IHC-P / Human
Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains
ARG66199: IHC-P / Human
Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice
ARG66199: IHC-P / Mouse
Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration
ARG66381: IHC-P / Mouse
"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis
ARG66381: WB / Mouse
ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis
ARG66381: ICC/IF / Human
VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.
ARG66381: IHC-P / Mouse
Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.
ARG66199: WB / Human
ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.
ARG66199: WB / Mouse
A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.
ARG66199: WB / Human
Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.
ARG66199: WB / Human
