ARG66195 anti-E Cadherin antibody [SQab1717] WB image

Western blot: 20 µg of MCF7 cell lysate stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:2000 dilution.

ARG66199 anti-Vimentin antibody [SQab1721] WB image

Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

ARG23870 anti-N Cadherin antibody WB image

Western blot: 20 µg of mouse brain stained with ARG23870 anti-N Cadherin antibody at 1:1000 dilution. (Observed MW: ~130 kDa)

ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image

Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 4°C. DAPI (blue) was used as the nuclear counter stain.

ARG23870 anti-N Cadherin antibody ICC/IF image

Immunofluorescence: MCF7-ADR cells stained with ARG23870 anti-N Cadherin antibody.

ARG66199 anti-Vimentin antibody [SQab1721] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

ARG66330 anti-GAPDH antibody [SQab1878] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human skin tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

ARG23870 anti-N Cadherin antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded human pancreatic adenocarcinoma stained with ARG23870 anti-N Cadherin antibody.

ARG66330 anti-GAPDH antibody [SQab1878] WB image

Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

ARG23870 anti-N Cadherin antibody WB image

Western blot: Human endothelial cells untreated (left) or treated with Pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23870 anti-N Cadherin antibody. (Observed MW: ~130 kDa)

ARG66199 anti-Vimentin antibody [SQab1721] IP image

Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

ARG66330 anti-GAPDH antibody [SQab1878] IP image

Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).

ARG23870 anti-N Cadherin antibody IP image

Immunoprecipitation: Mouse brain lysate immunoprecipitated with no antibody (lane 1), ARG23870 anti-N Cadherin antibody (lane 2) and whole mouse brain lysate (lane 3). The blot was stained with anti-N Cadherin (Cytoplasmic) Mouse monoclonal antibody (lanes 1-3).

ARG66199 anti-Vimentin antibody [SQab1721] WB image

Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human gastric adenocarcinoma tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

ARG66330 anti-GAPDH antibody [SQab1878] WB image

Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

ARG66330 anti-GAPDH antibody [SQab1878] WB image

Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].