ARG11065
anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9]
anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] for Indirect ELISA,ICC/IF,Western blot,Sandwich ELISA and Virus
Overview
| Product Description | Mouse Monoclonal antibody [IBDV9] recognizes IBDV / Gumboro disease virus VP3 |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | I-ELISA, ICC/IF, WB, sELISA |
| Specificity | Specific to VP3 protein of IBD virus. The antibody do not interact with other avian viruses. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IBDV9 |
| Isotype | IgG2a |
| Target Name | IBDV / Gumboro disease virus VP3 |
| Antigen Species | Virus |
| Immunogen | Purified very virulent IBD virus (field strain) derived from infected chicken bursas. |
| Conjugation | Un-conjugated |
| Alternate Names | Infectious bursal disease virus; Gumboro disease virus |
Application Instructions
| Application Suggestion |
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| Application Note | sELISA: Recommended antibody pairs in sandwich immunoassay (capture - detection): ARG11065 - ARG11068. Response strength of different IBDV strains: MB: - , S 706: +++ , GM 97: - , D 78: - , LC75: +++ . (+++ very high response, ++ high responce; + low responce; - no interaction) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4) and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
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ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] ICC image
Immunocytochemistry: Vero cells were cultured in 96-well plate, than infected with S706 IBDV strain and fixed with ethanol-acetone mixture. Sites of non-specific binding were blocked with 5% dry milk in PBS containing 0,1% Tween 20 (PBST).
Early stage of virus infection. After blocking cells were incubated 1 hour with ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9], conjugated with HRP, 1:500 dilution in PBST. After washing, 3,3'-diaminobenzidine HRP substrate was added. -
ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] WB image
Western blot: IBD virus stained with ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] in reducing condition.
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ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] sELISA image
sELISA: Capture Mab: ARG11065 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV9] (1 µg/well). Detection MAb (Eu-chelate labeled): ARG11068 anti-IBDV / Gumboro disease virus VP3 antibody [IBDV105] (0.4 µg/well). Incubation time: 30 min.
