ARG23074

anti-Collagen VII antibody [LH7.2]

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50 μg

315.00

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Overview

Product Description	Mouse Monoclonal antibody [LH7.2] recognizes Collagen VII Mouse anti Human collagen VII antibody, clone LH7.2 recognizes the carboxy-terminus of type VII collagen (Kirkham et al. 1989). Collagen VII is a basement membrane protein in stratified squamous epithelia which is involved in membrane organization through interaction with other ECM components (Gammon et al. 1992). It is composed of three identical alpha chains, each having an amino-terminal non-collagenous domain and a carboxy-terminal collagenous domain.Mouse anti Human collagen VII, clone LH7.2 has proved effective for immunohistochemical identification of basement membrane in human skin (Watson et al. 2001).
Tested Reactivity	Hu
Species Does Not React With	Ms, Rat, Pig
Tested Application	IHC-Fr
Host	Mouse
Clonality	Monoclonal
Clone	LH7.2
Isotype	IgG1
Target Name	Collagen VII
Antigen Species	Human
Immunogen	Crude extract of skin
Conjugation	Un-conjugated
Alternate Names	EBD1; EBR1; EBDCT; NDNC8; Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen

Application Instructions

Application Suggestion

Tested Application	Dilution
IHC-Fr	1:25 - 1:100

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein A.
Buffer	PBS and 0.09% Sodium azide
Preservative	0.09% Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1294 Human COL7A1 Swiss-port # Q02388 Human Collagen alpha-1(VII) chain
Gene Symbol	COL7A1
Gene Full Name	collagen, type VII, alpha 1
Background	This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]
Function	Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. [UniProt]
Calculated MW	295 kDa
PTM	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Images (1) Click the Picture to Zoom In

ARG23074 anti-Collagen VII antibody [LH7.2] IHC-Fr image

Immunohistochemistry: Human ileum cryosection stained with ARG23074 anti-Collagen VII antibody [LH7.2].