ARG30263
Mitochondrial fission Antibody Duo (Drp1, BAX)
Publication5
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG55200 | anti-DNM1L / DRP1 antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG65612 | anti-Bax antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
Overview
| Product Description | Mitochondria routinely undergo fission and fusion events that maintain a dynamic reticular network. Dynamin-1-like protein (DNM1L), also known as Drp1, is a GTPase that fundamentally regulates mitochondrial fission. In response to many apoptotic stimuli, oligomerization of Bax is essential for mitochondrial outer membrane permeabilization and the ensuing release of cytochrome c. These events are accompanied by mitochondrial fission that require Drp1. This antibody duo provides useful tool for the study of the mechanism of Bax oligomerization in apoptosis and other biological processes. Montessuit et al. (2010) Cell (142): 889-901 |
|---|---|
| Target Name | Mitochondrial fission |
| Alternate Names | Mitochondrial fission antibody; DNM1L / DRP1 antibody; Bax antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (9) Click the Picture to Zoom In
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ARG65612 anti-Bax antibody WB image
Western blot: 30 µg of MCF-7, HCT116, U87-MG, and HepG2 cell line lysates stained with ARG65612 anti-Bax antibody at 1:500 dilution.
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ARG65612 anti-Bax antibody ICC/IF image
Immunocytochemistry: HeLa cells fixed with Methanol / Acetone 1:1 ratio (-20°C for 20 min) and blocked by 3% BSA in PBS (RT for 1 hour). The cells stained with ARG65612 anti-Bax antibody at 1:20 dilution (RT for 1 hour).
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ARG55200 anti-DNM1L / DRP1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55200 anti-DNM1L / DRP1 antibody.
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ARG55200 anti-DNM1L / DRP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG55200 anti-DNM1L / DRP1 antibody at 1:250 dilution.
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ARG65612 anti-Bax antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human esophagus cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG65612 anti-Bax antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human lung cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG55200 anti-DNM1L / DRP1 antibody WB image
Western blot: Raji cell lysate stained with ARG55200 anti-DNM1L / DRP1 antibody.
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ARG65612 anti-Bax antibody WB image
Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG65612 anti-Bax antibody at 1:3000 dilution.
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ARG55200 anti-DNM1L / DRP1 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG55200 anti-DNM1L / DRP1 antibody at 1:8000 dilution.
Specific References
Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions
ARG65612: WB / Human
Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers
ARG65612: WB / Human
Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling
ARG65612: WB / Human
Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.
ARG65612: WB / Rat
A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis
ARG65612: WB / Mouse
