ARG30268

Apoptosis Marker Antibody Duo (Bcl2, Bax)

Datasheet

Publication25

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1 pair

459.00

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Datasheet
Publication (25)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG65612	anti-Bax antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, WB	50 μl
ARG55188	anti-Bcl 2 antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IP, WB	50 μl

Overview

Product Description	BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax protein belongs to the BCL2 protein family. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. (provided by RefSeq, Jul 2008) BAX/BCL2 expression ratio has been identified as an import index to figure out the fate of the cell undergoing apoptosis or anti-apoptosis in variety studies, including cancer study, therapeutic agent searching and therapeutic response evaluation. arigo provide an Apoptosis marker Duo, ARG30268, including anti-Bcl2 and anti-Bax antibodies, is useful for user in studying BAX/BCL2 correlation.
Target Name	Apoptosis Marker
Alternate Names	Apoptosis Marker antibody; Bcl 2 antibody; Bax antibody

Product Description

BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.

Bax protein belongs to the BCL2 protein family. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. (provided by RefSeq, Jul 2008)

BAX/BCL2 expression ratio has been identified as an import index to figure out the fate of the cell undergoing apoptosis or anti-apoptosis in variety studies, including cancer study, therapeutic agent searching and therapeutic response evaluation.

arigo provide an Apoptosis marker Duo, ARG30268, including anti-Bcl2 and anti-Bax antibodies, is useful for user in studying BAX/BCL2 correlation.

Target Name

Apoptosis Marker

Alternate Names

Apoptosis Marker antibody; Bcl 2 antibody; Bax antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Images (7) Click the Picture to Zoom In

ARG65612 anti-Bax antibody WB image

Western blot: 30 µg of MCF-7, HCT116, U87-MG, and HepG2 cell line lysates stained with ARG65612 anti-Bax antibody at 1:500 dilution.
ARG65612 anti-Bax antibody ICC/IF image

Immunocytochemistry: HeLa cells fixed with Methanol / Acetone 1:1 ratio (-20°C for 20 min) and blocked by 3% BSA in PBS (RT for 1 hour). The cells stained with ARG65612 anti-Bax antibody at 1:20 dilution (RT for 1 hour).
ARG55188 anti-Bcl 2 antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG55188 anti-Bcl 2 antibody (red) at 1:100 dilution. DAPI (blue) for nuclear staining.
ARG65612 anti-Bax antibody IHC-P image

Immunohistochemistry: paraffin-embedded Human esophagus cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
ARG65612 anti-Bax antibody WB image

Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG65612 anti-Bax antibody at 1:3000 dilution.
ARG55188 anti-Bcl-2 antibody WB image

Western blot: 30 μg of Jurkat cell lysate stained with ARG55188 anti-Bcl-2 antibody at 1:1000 dilution.
ARG65612 anti-Bax antibody IHC-P image

Immunohistochemistry: paraffin-embedded Human lung cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)

Specific References

Transcutaneous electrical acupoint stimulation alleviates cerebral ischemic injury through the TLR4/MyD88/NF-κ B pathway

ARG55188: WB / Rat

Front Cell Neurosc. et al.
Front Cell Neurosci., (2024)

Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes

ARG55188: WB / Human

Limin Zhang et al.
Heliyon., (2024)

Probucol attenuates high glucose-induced Müller cell damage through enhancing the Nrf2/p62 signaling pathway

ARG55188: WB / Human

Yu-Fan Zhou et al.
Int Ophthalmol., (2023)

Dapagliflozin protects against doxorubicin-induced nephrotoxicity associated with nitric oxide pathway-A translational study

ARG55188: WB / Rat

Wei-Ting Chang et al.
Free Radic Biol Med., (2023)

Astaxanthin alleviates fine particulate matter (PM2.5)-induced lung injury in rats by suppressing ferroptosis and apoptosis

ARG55188: WB / Rat

Bowen Yin et al.
Food & Function., (2023)

Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage

ARG55188: WB / Rat

Xuecheng Zheng et al.
Chin Med J (Engl)., (2023)

Bone targeted miRNA delivery system for miR-34a with enhanced anti-tumor efficacy to bone-associated metastatic breast cancer

ARG55188: WB / Human

Tian-Yan Han et al.
Int J Pharm., (2023)

Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells

ARG55188: WB / Human

Ying Liang et al.
Gynecol Endocrinol., (2023)

Dapagliflozin-entresto protected kidney from renal hypertension via downregulating cell-stress signaling and upregulating SIRT1/PGC-1α/Mfn2-medicated mitochondrial homeostasis

ARG55188: WB / Rat

Sheung-Fat Ko et al.
Exp Biol Med (Maywood), (2023)

Ivabradine could not decrease mitral regurgitation triggered atrial fibrosis and fibrillation compared with carvedilol

ARG55188: WB / Rat

Wei-Chieh Lee et al.
ESC Heart Fail., (2023)

Transpulmonary Expression of Exosomal microRNAs in Idiopathic and Congenital Heart Disease-Related Pulmonary Arterial Hypertension

ARG55188: WB / Human

Wei-Ting Chang et al.
J Am Heart Assoc, (2023)

Deletion of MicroRNA-21 Impairs Neovascularization Following Limb Ischemia: From Bedside to Bench

ARG55188: WB / Mouse

Wei-Ting Chang et al.
Front Cardiovasc Med., (2022)

Naringin Interferes Doxorubicin-Induced Myocardial Injury by Promoting the Expression of ECHS1

ARG55188: WB / Rat

Zirui Zhao et al.
Front Pharmacol., (2022)

Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

ARG55188, ARG65612: WB / Human

Tzu-Chun Kan et al.
Pharmaceutics., (2022)

Orlistat Resensitizes Sorafenib-Resistance in Hepatocellular Carcinoma Cells through Modulating Metabolism

ARG55188:WB / Human

Pei-Wei Shueng et al.
International Journal of Molecular Sciences, (2022)

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

ARG65612: WB / Human

Peng-Hsiang Fang et al.
Molecular Medicine, (2022)

Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

ARG65612: WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci., (2021)

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG55188: WB / Human

Jing Wang et al.
Arch Oral Biol., (2021)

Dapagliflozin Improves Cardiac Hemodynamics and Mitigates Arrhythmogenesis in Mitral Regurgitation-Induced Myocardial Dysfunction.

ARG55188: WB / Rat

Yu-Wen Lin et al.
J Am Heart Assoc., (2021)

miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI

ARG55188: WB / Human

Xue Luo et al.
Biosci Rep., (2020)

Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.

ARG65612: WB / Rat

Zhonghua Li et al.
Behav Brain Res., (2020)

Xenogeneic and Allogeneic Mesenchymal Stem Cells Effectively Protect the Lung Against Ischemia-reperfusion Injury Through Downregulating the Inflammatory, Oxidative Stress, and Autophagic Signaling Pathways in Rat.

ARG55188: WB / Human

Kun-Chen Lin et al.
Cell Transplant., (2020)

Possible Involvement of PTEN Signaling Pathway in the Anti-apoptotic Effect of Electroacupuncture Following Ischemic Stroke in Rats

ARG55188: WB / Rat

Xing Ying et al.
Cell Mol Neurobiol., (2018)

Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.

ARG55188: WB / Rat

Xing Ying et al.
J Mol Neurosci., (2018)

A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

ARG55188, ARG65612: WB / Mouse

Xuan Wang et al.
Oncol Lett., (2018)