How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer.
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer.
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Most vertebrates have limbs except snakes. A group in Lawrence Berkeley National Laboratory is highly interested in the reason why snakes lose the limbs. Dr. Kvon and colleagues recently reported that the critical ZRS limb enhancer has progressively lost its function leading to limb loss during snake evolution.
It was found that the activity of evolutionarily conserved ZRS limb enhancer is lost in snake. The authors employed CRISPR/Cas9 genome editing to generate a series of knockin mice where the ZRS was replaced with the ZRS orthologous from other vertebrate species. Only snake enhancer knockin causes severe limb truncation in mice. The authors further identified a 17-bp snake-specific deletion in ZRS. This 17-bp sequence is able to resurrect snake ZRS function and rescue the limb truncation phenotype in snake ZRS-knockin mice. arigo is proud to introduce our new Cas9 antibodies that are from multiple host species. All are good for checking Cas9 expression level by WB and localization of Cas9 protein via ICC or IHC.
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Mouse monoclonal antibody |
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Chicken polyclonal antibody |
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Chicken polyclonal antibody |
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Reference:
Kvon, E.Z. et al., (2016) Progressive Loss of Function in a Limb Enhancer during Snake Evolution. Cell. 167:633-42.
