ARG83583
arigoQIKTM Human TNF alpha ELISA Development Kit
arigoQIKTM Human TNF alpha ELISA Development Kit for ELISA and Human
Overview
| Product Description | ARG83583 arigoQIKTM Human TNF alpha ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution. This ELISA Development Kit is designed for the development of sandwich ELISA to measure Human TNF alpha in Serum, plasma and cell culture supernatants. For other reagents required for arigoQIKTM ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit) More about arigoQIKTM: ● Optimized capture and detection antibody pairs ● Reduced incubation time and wash cycles ● 2-hour quicker than conventional ELISA process ● 5- and 15-plate packages available |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | TNF alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 50 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | TNF; Tumor Necrosis Factor; TNF-Alpha; TNFSF2; TNFA; DIF 2; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-A; Tumor Necrosis Factor (TNF Superfamily, Member 2); Tumor Necrosis Factor Ligand 1F; Tumor Necrosis Factor-Alpha; Tumor Necrotic Factor Alpha; TNF Superfamily, Member 2; TNF, Macrophage-Derived; TNF, Monocyte-Derived; APC1 Protein; Cachectin; TNLG1F |
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNF |
| Gene Full Name | Tumor Necrosis Factor |
| Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020] |
| Function | Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Promotes osteoclastogenesis and therefore mediates bone resorption. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Secreted. [UniProt] |
| PTM | Disulfide bond, Glycoprotein, Lipoprotein, Myristate, Phosphoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83583 arigoQIK Human TNF alpha ELISA Development Kit standard curve image
ARG83583 arigoQIK Human TNF alpha ELISA Development Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83583 arigoQIKTM Human TNF alpha ELISA Development Kit User manual |
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