ARG83356
arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha)
arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha) for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG83356-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83356-02 | Standard Mixture | 3 vials | 4°C |
| ARG83356-03 | 10X Antibody Conjugate Mixture | 1.2 ml | ≤ -20°C |
| ARG83356-04 | 40X Streptavidin-HRP concentrate | 300 μl | 4°C |
| ARG83356-05 | Standard/Sample Diluent buffer | 35 ml | 4°C |
| ARG83356-06 | Antibody Diluent buffer | 30 ml | 4°C |
| ARG83356-07 | 10X Wash Buffer | 60 ml | 4°C |
| ARG83356-08 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83356-09 | STOP solution | 12 ml | 4°C |
| ARG83356-10 | Plate sealer | 4 adhesive strips | Room temperature |
Overview
| Product Description | ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha) is an Enzyme Immunoassay kit for the quantification of TGF beta 1, PDGF BB, IL6, TNF alpha in serum, plasma and cell culture supernatant. See all Multiplex ELISA kits |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | Rat Fibrotic Marker |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | TGF beta1: 15.625 pg/mL PDGF BB: 15.625 pg/mL IL6: 31.25 pg/mL TNF alpha: 15.625 pg/mL |
| Sample Type | Serum, Plasma and Cell culture supernatant. |
| Standard Range | TGF beta1: 31.25 - 2000 pg/mL PDGF BB: 31.25 - 2000 pg/mL IL6: 62.5 ~ 4000 pg/mL TNF alpha: 31.25 - 2000 pg/mL |
| Sample Volume | 50 µl |
Application Instructions
| Assay Time | 4.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TGFB1; PDGFB; IL6; TNF |
|---|---|
| Gene Full Name | transforming growth factor, beta 1; platelet-derived growth factor beta polypeptide; interleukin 6; tumor necrosis factor |
| Function | TGF beta 1: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. PDGF BB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. IL6: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). TNF alpha: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. |
| Highlight | Related Product: TGF beta 1 antibodies; PDGF BB antibodies; IL6 antibodies; TNF alpha antibodies; Related news: Fibrotic Marker Multiplex ELISA Kits are launch; |
Images (5) Click the Picture to Zoom In
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Antibodies Coating Pattern In Microtiter Plate of ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha)
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ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image
ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Rat TGF beta 1 run with optical density reading at 450 nm.
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ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image
ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Rat PDGF BB run with optical density reading at 450 nm.
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ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image
ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Rat IL6 run with optical density reading at 450 nm.
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ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image
ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Rat TNF alpha run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83356 arigoPLEX® Rat Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha) User manual |
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