ARG83474

arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1, TNF alpha)

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96 wells

1,149.00

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Product Description	ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha) is an Enzyme Immunoassay kit for the quantification of GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP1 and TNF alpha in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
Tested Reactivity	Hu
Tested Application	ELISA
Target Name	M1/M2/MDSC Cytokine
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	GM-CSF: 7.8 pg/ml IFN gamma: 7.8 pg/ml IL4: 7.8 pg/ml IL6: 15.6 pg/ml IL10: 7.8 pg/ml IL12: 15.6 pg/ml MCP1: 15.6 pg/ml TNF alpha: 15.6 pg/ml
Sample Type	Serum, Plasma and Cell culture supernatants
Standard Range	GM - CSF: 15.6 - 500 pg/ml IFN gamma: 15.6 - 500 pg/ml IL4: 15.6 - 500 pg/ml IL6: 31.25 - 1000 pg/ml IL10: 15.6 - 500 pg/ml IL12: 31.25 - 1000 pg/ml MCP1: 31.25 - 1000 pg/ml TNF alpha: 31.25 - 1000 pg/ml
Sample Volume	50 µl
Alternate Names	GM-CSF: CSF2; Colony Stimulating Factor 2; GMCSF; Sargramostim; GM-CSF; Colony Stimulating Factor 2 (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor; Molgramostim; Molgramostin; CSF; Granulocyte-Macrophage Colony Stimulating Factor; Granulocyte Macrophage-Colony Stimulating Factor; Colony-Stimulating Factor IFN gamma: IFNG; Interferon Gamma; Immune Interferon; IFN-Gamma; IMD69; IFG; IFI IL4: IL4; Interleukin 4; IL-4; Lymphocyte Stimulatory Factor 1; BCGF-1; BCGF1; BSF1; B_cell Stimulatory Factor 1; B Cell Growth Factor 1; Interleukin-4; Binetrakin; Pitrakinra; MGC79402; BSF-1; B-Cell Stimulatory Factor 1 IL6: IL6; Interleukin 6; IL-6; IFNB2; BSF2; HGF; HSF; B-Cell Stimulatory Factor 2; CTL Differentiation Factor; Hybridoma Growth Factor; Interferon Beta-2; Interleukin-6; IFN-Beta-2; BSF-2; CDF; Interleukin 6 (Interferon, Beta 2); B-Cell Differentiation Factor; Interferon, Beta 2; Interleukin BSF-2 IL10: IL10; Interleukin 10; IL-10; CSIF; Cytokine Synthesis Inhibitory Factor; IL10A; TGIF; T-Cell Growth Inhibitory Factor; Interleukin-10; GVHDS IL12: IL12A; Interleukin 12A; IL-12A; NKSF1; CLMF; NFSK; P35; Interleukin 12A (Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, P35); Cytotoxic Lymphocyte Maturation Factor 35 KDa Subunit; Cytotoxic Lymphocyte Maturation Factor 1, P35; NF Cell Stimulatory Factor Chain 1; NK Cell Stimulatory Factor Chain 1; Interleukin-12 Subunit Alpha; Interleukin-12 Alpha Chain; Interleukin 12, P35; IL-12, Subunit P35; IL35 Subunit; CLMF P35; Natural Killer Cell Stimulatory Factor 1, 35 KD Subunit ; IL-12 Subunit P35 MCP1: CCL2; C-C Motif Chemokine Ligand 2; MCP-1; MCP1; MCAF; HC11; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE; SMC-CF; GDCF-2; SCYA2; Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je); Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Monocyte Chemoattractant Protein-1; Chemokine (C-C Motif) Ligand 2; Monocyte Chemotactic Protein 1; Small-Inducible Cytokine A2; C-C Motif Chemokine 2; MGC9434; Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je; Monocyte Chemoattractant Protein 1; HSMCR30 TNF alpha: TNF; Tumor Necrosis Factor; TNF-Alpha; TNFSF2; TNFA; DIF 2; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-A; Tumor Necrosis Factor (TNF Superfamily, Member 2); Tumor Necrosis Factor Ligand 1F; Tumor Necrosis Factor-Alpha; Tumor Necrotic Factor Alpha; TNF Superfamily, Member 2; TNF, Macrophage-Derived; TNF, Monocyte-Derived; APC1 Protein; Cachectin; TNLG1F

Application Instructions

Assay Time	4.5 hour

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background	GM-CSF: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020] IFN gamma: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] IL4: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020] IL6: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020] IL10: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] IL12: This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008] MCP1: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. [provided by RefSeq, Aug 2020] TNF alpha: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]
Highlight	Related news: M1/M2 Cytokine Multiplex ELISA Kit is launched;

Background

GM-CSF: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]

IFN gamma: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

IL4: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]

IL6: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]

IL10: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]

IL12: This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

MCP1: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. [provided by RefSeq, Aug 2020]

TNF alpha: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]

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Images (9) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit (GM-CSF, IFN gamma, IL4, IL6, IL10, IL12, MCP-1, TNF alpha)
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human GM-CSF run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human IL4 run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human IL6 run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human IL10 run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human IL12 run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human MCP1 run with optical density reading at 450 nm.
ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit standard curve image

ARG83474 arigoPLEX® Human M1/M2/MDSC Cytokine Multiplex ELISA Kit results of a typical standard for Human TNF alpha run with optical density reading at 450 nm.