ARG55944
anti-p27 Kip1 antibody [SX53G8]
anti-p27 Kip1 antibody [SX53G8] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [SX53G8] recognizes p27 Kip1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SX53G8 |
| Isotype | IgG1, kappa |
| Target Name | p27 Kip1 |
| Antigen Species | Human |
| Immunogen | Purified fusion protein of Human p27 Kip1. |
| Conjugation | Un-conjugated |
| Alternate Names | Cyclin-dependent kinase inhibitor 1B; MEN4; KIP1; P27KIP1; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN4; MEN1B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P46414 Mouse Cyclin-dependent kinase inhibitor 1B Swiss-port # P46527 Human Cyclin-dependent kinase inhibitor 1B |
|---|---|
| Gene Symbol | CDKN1B |
| Gene Full Name | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
| Background | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014] |
| Function | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. [UniProt] |
| Cellular Localization | Nuclear |
| Calculated MW | 22 kDa |
| PTM | Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation. Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG55944 anti-p27 Kip1 antibody [SX53G8] FACS image
Flow Cytometry: HeLa cells stained with Alexa Fluor 488-conjugated ARG55944 anti-p27 Kip1 antibody [SX53G8] (green) and isotype control antibody (gray).
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ARG55944 anti-p27 Kip1 antibody [SX53G8] IHC-P image
Immunohistochemistry: Human colon stained with ARG55944 anti-p27 Kip1 antibody [SX53G8].
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ARG55944 anti-p27 Kip1 antibody [SX53G8] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded prostate carcinoma stained with ARG55944 anti-p27 Kip1 antibody [SX53G8].
Customer's Feedback
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Review for anti-p27 Kip1 antibody [SX53G8]
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：0.5 μg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
