ARG56509

anti-iNOS antibody

anti-iNOS antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal

Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
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publication_link Publication19

Overview

Product Description Rabbit Polyclonal antibody recognizes iNOS
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity This antibody may cross-react to nNOS (~5%) and does not cross-react to eNOS.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name iNOS
Antigen Species Mouse
Immunogen Purified enzyme from Mouse macrophages (RAW 264.7) cells.
Conjugation Un-conjugated
Alternate Names HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IHC-FrAssay-dependent
IHC-P1:200 - 1:1000
IP1:200
WB1:200 - 1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 or Tris-EDTA pH 9.0 for 20 min followed by cooling at RT for 20 min. Incubate with the antibody at 4°C for overnight.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer TBS (pH 7.4) and 0.5 mg/ml BSA.
Stabilizer 0.5 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18126 Mouse NOS2

GeneID: 24599 Rat NOS2

GeneID: 4843 Human NOS2

Gene Symbol Nos2
Gene Full Name nitric oxide synthase 2, inducible
Background iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Function iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30323 Inflammation Antibody Panel
ARG30333 M1/M2/TAM Marker Antibody Panel
Related products:
iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
Calculated MW 131 kDa

Images (5) Click the Picture to Zoom In

  • ARG56509 anti-iNOS antibody IHC-P image

    Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.

    From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.

  • ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)

    Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG56509 anti-iNOS antibody WB image

    Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.

    From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.

  • ARG56509 anti-iNOS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.

  • ARG56509 anti-iNOS antibody WB image

    Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.

Customer's Feedback

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Review for anti-iNOS antibody

Application:IF/ICC

Sample:RAW264

Fixation Buffer:4% PFA

Fixation Time:15 min

Fixation Temperature:Room Temperature ºC

Permeabilization Buffer:4% PFA

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:4 hr

Primary Antibody Incubation Temperature:Room Temperature ºC

nuts_pic      Excellent

Review for anti-iNOS antibody

Application:WB

Sample:Raw264.7 untreated or treated with LPS

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:400

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Effect of macrophage-to-myofibroblast transition on silicosis

IHC-P,WB / Rat

Fei Geng et al.
Anim Models Exp Med.,  (2024)

publication_link

 

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Aquaporin-9 Aggravates Lipopolysaccharides Induced Acute Lung Injury Via Facilitating M1-Like Macrophage Polarization

IHC-P / Mouse

Jiajia Wan et al.
preprint,  (2024)

publication_link

 

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Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair

IHC-P / Mouse

Xu Zhang et al.
Bioact Mater.,  (2024)

publication_link

 

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Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice

WB, IHC-P / Mouse

Xin-Yue Yang et al.
J Ethnopharmacol.,  (2024)

publication_link

 

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Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice

IHC-P / Mouse

Zhenyu Feng et al.
Heliyon,  (2024)

publication_link

 

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Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration

ICC/IF / Human

Zeling Huang et al.
Heliyon.,  (2024)

publication_link

 

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Quercetin Alleviates Pulmonary Fibrosis in Silicotic Mice by Inhibiting Macrophage Transition and TGF-β-Smad2/3 Pathway

WB / Mouse

Fei Geng et al.
Curr. Issues Mol. Biol,  (2023)

publication_link

 

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Modulating phenylalanine metabolism by L. acidophilus alleviates alcohol-related liver disease through enhancing intestinal barrier function

IHC-P / Mouse

Liuying Chen et al.
Cell Biosci.,  (2023)

publication_link

 

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Anti-Inflammatory Actions of G-Protein-Coupled Estrogen Receptor 1 (GPER) and Brain-Derived Estrogen Following Cerebral Ischemia in Ovariectomized Rats

WB/IHC-Fr / Rat

Jing Xu et al.
Biology (Basel).,  (2023)

publication_link

 

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Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

WB / Rat

Wahid Shah et al.
Sci Rep.,  (2023)

publication_link

 

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Evaluation Of The Histopathological, Immunohistochemical, And Oxidative Neurotoxic Effects of Nanoaluminum Present In Human Vaccines In Newborn Albino Rats

IHC-P / Rat

Abrar Roshdy Abouelkeir et al.
European Chemical Bulletin,  (2023)

publication_link

 

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Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

IHC-P / Rat

Man Wang et al.
BMC Complement Med Ther,  (2022)

publication_link

 

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Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia

ICC/IF / Mouse

Miao Tang et al.
Pharm Biol.,  (2022)

publication_link

 

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Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants

ICC/IF / Mouse

Sunil Kumar Boda. et al.
Biomater Sci.,  (2022)

publication_link

 

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Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment

WB / Rat

Na Mao et al.
Int J Mol Sci.,  (2021)

publication_link

 

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Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.

WB / Mouse

Yajing Chen et al.
J Ethnopharmacol.,  (2020)

publication_link

 

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A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.

IHC-P / Rat

Tang Qian et al.
Biomaterials.,  (2020)

publication_link

 

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HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.

IHC-P / Mouse

Motterlini Roberto et al.
Redox Biol.,  (2018)

publication_link

 

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Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis

IHC-P / Rat

Liang Hongling et al.
口腔疾病防治,  (2018)

publication_link

 

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