ARG56509
anti-iNOS antibody
anti-iNOS antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal
Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
Publication15
Overview
| Product Description | Rabbit Polyclonal antibody recognizes iNOS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | This antibody may cross-react to nNOS (~5%) and does not cross-react to eNOS. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | iNOS |
| Antigen Species | Mouse |
| Immunogen | Purified enzyme from Mouse macrophages (RAW 264.7) cells. |
| Conjugation | Un-conjugated |
| Alternate Names | HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 or Tris-EDTA pH 9.0 for 20 min followed by cooling at RT for 20 min. Incubate with the antibody at 4°C for overnight. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | TBS (pH 7.4) and 0.5 mg/ml BSA. |
| Stabilizer | 0.5 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Nos2 |
| Gene Full Name | nitric oxide synthase 2, inducible |
| Background | iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| Function | iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30323 Inflammation Antibody Panel ARG30333 M1/M2/TAM Marker Antibody Panel Related products: iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| Research Area | Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody |
| Calculated MW | 131 kDa |
Images (5) Click the Picture to Zoom In
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ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.
From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.
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ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)
Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG56509 anti-iNOS antibody WB image
Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.
From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.
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ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.
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ARG56509 anti-iNOS antibody WB image
Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.
Customer's Feedback
Excellent
Review for anti-iNOS antibody
Application：IF/ICC
Sample：RAW264
Fixation Buffer：4% PFA
Fixation Time：15 min
Fixation Temperature：Room Temperature ºC
Permeabilization Buffer：4% PFA
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：4 hr
Primary Antibody Incubation Temperature：Room Temperature ºC
Excellent
Review for anti-iNOS antibody
Application：WB
Sample：Raw264.7 untreated or treated with LPS
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:400
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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