ARG58646
anti-eEF1D antibody
anti-eEF1D antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eEF1D |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eEF1D |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-160 of Human eEF1D (NP_001123529.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Antigen NY-CO-4; FP1047; EF-1-delta; EF1D; EF-1D; Elongation factor 1-delta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | A549 | ||||||||||
| Observed Size | 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EEF1D |
| Gene Full Name | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| Background | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010] |
| Function | Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 31 kDa |
Images (3) Click the Picture to Zoom In
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ARG58646 anti-eEF1D antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58646 anti-eEF1D antibody.
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ARG58646 anti-eEF1D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58646 anti-eEF1D antibody at 1:200 dilution.
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ARG58646 anti-eEF1D antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG58646 anti-eEF1D antibody at 1:1000 dilution.
