ARG62683
anti-beta III Tubulin antibody [TU-20]
anti-beta III Tubulin antibody [TU-20] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Baboon,Bovine,Broad,Carp,Chicken,Hamster,Pig
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [TU-20] recognizes beta III Tubulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bb, Bov, Broad, Carp, Chk, Hm, Pig |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | The clone TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III Tubulin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TU-20 |
| Isotype | IgG1 |
| Target Name | beta III Tubulin |
| Immunogen | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide. |
| Conjugation | Un-conjugated |
| Alternate Names | CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: In reducing conditions, incubated for 90 min. Positive control: Porcine brain lysate; Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. IHC-P: Staining technique: Standard ABC technique (DAB+). Antigen Retrieval: Heat the tissue section in 10 mM Citrate buffer (pH 6.0) or pretreat samples with 0.1% pepsin (trypsin) in 0.1M HCl for 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Positive tissue: neuronal tissue Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by ammonium sulphate and caprylic acid precipitation |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBB3 |
| Gene Full Name | tubulin, beta 3 class III |
| Background | The beta III-Tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-Tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII) ARG30301 Neurite Marker Antibody Duo Related products: beta III Tubulin antibodies; beta III Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody |
| Calculated MW | 50 kDa |
| PTM | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
Images (6) Click the Picture to Zoom In
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ARG62683 anti-beta III Tubulin antibody [TU-20] WB image
Western blot: 30 µg of 1) HeLa (negative control), 2) Mouse brain, and 3) Rat brain lysate stained with ARG62683 anti-beta III Tubulin antibody [TU-20] at 1:1000 dilution.
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ARG62683 anti-beta III Tubulin antibody [TU-20] IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG62683 anti-beta III Tubulin antibody [TU-20].
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ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image
Immunofluorescence: Neuro2a mouse neuroblastoma cell stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (green).
Cell nuclei was stained with DAPI (blue). -
ARG62683 anti-beta III Tubulin antibody [TU-20] IHC-P image
Immunohistochemistry: Mouse brain stained with ARG62683 anti-beta III Tubulin antibody [TU-20].
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ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image
Immunofluorescence: P-19 mouse embryonal carcinoma cells stimulated to neuronal differentiation by retinoic acid stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red).
Cell nuclei was stained with DAPI (blue). -
ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image
Immunofluorescence: P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid co-stained with stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red) and anti-beta-tubulin (green)
Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
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Review for anti-beta III Tubulin antibody [TU-20]
Application：WB
Sample：1) HeLa (negative control), 2) Mouse brain, 3) Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.
WB, IHC-Fr / Rat
