ARG62675
anti-beta Endorphin antibody [B31.15]
anti-beta Endorphin antibody [B31.15] for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [B31.15] recognizes beta Endorphin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-Fr, IHC-P |
| Specificity | The clone B31.15 reacts with human beta Endorphin, an endogenous opiate derived from ACTH gene. ACTH (Corticotropin; human 39 aa) is synthesized by the anterior pituitary gland and stimulates the adrenal cortex; 6 hormones are derived from one ACTH gene: ACTH, lipotropin, alpha-MSH, beta-MSH, endorphin, and one other. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B31.15 |
| Isotype | IgG1 |
| Target Name | beta Endorphin |
| Antigen Species | Human |
| Immunogen | Human beta Endorphin (full length native protein). |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-MSH; Beta-MSH; CLIP; Gamma-MSH; LPH; Corticotropin-lipotropin; NPP; ACTH; POMC; Gamma-LPH; Adrenocorticotropic hormone; MSH; Beta-LPH; Pro-opiomelanocortin; POC |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Pretreatment: Heat retrieval (microwave oven). Staining technique: Standard ABC technique (DAB+). Incubation: overnight at 4°C. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Human pituitary gland |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POMC |
| Gene Full Name | proopiomelanocortin |
| Background | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Nov 2014] |
| Function | ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. [UniProt] |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 29 kDa |
| PTM | Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine. |
Images (1) Click the Picture to Zoom In
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ARG62675 anti-beta Endorphin antibody [B31.15] IHC-Fr image
Immunohistochemistry: Human pituitary gland (frozen sections) stained with ARG62675 anti-beta Endorphin antibody [B31.15].
Clone References
Gonadotropin-inhibitory hormone identification, cDNA cloning, and distribution in rhesus macaque brain.
Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage.
