ARG52485
anti-alpha smooth muscle Actin antibody [1A4]
anti-alpha smooth muscle Actin antibody [1A4] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig,Rabbit,Sheep
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
Publication9
Overview
| Product Description | Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin (SMA) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig, Rb, Sheep |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A4 |
| Isotype | IgG1, kappa |
| Target Name | alpha smooth muscle Actin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. |
| Conjugation | Un-conjugated |
| Alternate Names | Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Incubation time: overnight at 4°C or 30-60 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Leiomyoma, Small Intestine (Smooth Muscle) |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained. |
| Cellular Localization | Cytoplasm |
| Highlight | Related Antibody Duos and Panels: ARG30293 Smooth Muscle Marker Related products: alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs Besides tumor suppression, what’s p53 busy for during embryogenesis? New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody |
| Calculated MW | 42 kDa |
| PTM | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
Images (3) Click the Picture to Zoom In
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ARG52485 anti-alpha smooth muscle Actin antibody [1A4] ICC/IF image
Immunofluorescence: Rabbit skeletal muscle stained with ARG52485 anti-alpha smooth muscle Actin antibody [1A4] at 1:100 dilution.
Secondary antibody: ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed
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ARG52485 anti-alpha smooth muscle Actin antibody [1A4] WB image
Western blot: 30 µg of MEF cell lysate stained with anti-alpha smooth muscle Actin antibody [1A4] at 1:200 dilution.
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ARG52485 anti-Actin-Smooth Muscle antibody [1A4] IHC-P image
Immunohistochemistry: Human Small Intestine stained with Actin-Smooth Muscle antibody [1A4] (ARG52485)
Customer's Feedback
Excellent
Review for anti-alpha smooth muscle Actin antibody [1A4]
Application：IF/ICC
Sample：Rabbit skeletal muscle
Fixation Buffer：4% PFA
Fixation Time：20 min
Fixation Temperature：25 ºC
Antigen Retrieval Buffer：-
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-alpha smooth muscle Actin antibody [1A4]
Application：WB
Sample：Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:300
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-alpha smooth muscle Actin antibody [1A4]
Application：WB
Sample：MEF cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Comparative Assessment of Short-Term Tendon-Scleral Postoperative Inflammation and α-Smooth Muscle Actin Expression following Oral and Topical Diclofenac Administration for Strabismus Surgery in Rabbits
IHC-P / Rabbit
Pleiotropic Long-Term Effects of Atorvastatin on Posttraumatic Joint Contracture in a Rat Model
IHC-P / Rat
Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft.
IHC-P / Rabbit
MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.
IHC-P / Human
Inhibiting necroptosis of spermatogonial stem cell as a novel strategy for male fertility preservation.
IHC-P / Mouse
Effects of losartan and atorvastatin on the development of early posttraumatic joint stiffness in a rat model.
IHC-P / Rat
Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection.
IHC-P / Mouse
Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability
IHC-P / Mouse
Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy
IHC-P / Rat
