ARG22797

anti-alpha Tubulin antibody [YOL1/34]

anti-alpha Tubulin antibody [YOL1/34] for ELISA,ICC/IF,IHC-Frozen sections,Radioimmunoassay,Western blot and Human,Mouse,Rat,Arabidopsis,Bird,D. melanogaster ,Fungi,Mammal,Yeast

Signaling Transduction antibody; Loading Control antibody

Overview

Product Description Rat Monoclonal antibody [YOL1/34] recognizes alpha Tubulin
This antibody recognizes alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2.
Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin.
Tested Reactivity Hu, Ms, Rat, Arabi, Bird, Dm, Fungi, Mamm, Yeast
Tested Application ELISA, ICC/IF, IHC-Fr, RIA, WB
Host Rat
Clonality Monoclonal
Clone YOL1/34
Isotype IgG2a
Target Name alpha Tubulin
Antigen Species Yeast
Immunogen Yeast tubulin
Epitope This antibody binds to an epitope between amino acids 414 and 422 of alpha tubulin (EEGEFTEAR).
Conjugation Un-conjugated
Alternate Names Tubulin K-alpha-1; Alpha-tubulin ubiquitous; Tubulin alpha-ubiquitous chain; Tubulin alpha-1B chain; K-ALPHA-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA10 µg/ml (detecting Ab)
ICC/IFAssay-dependent
IHC-FrAssay-dependent
RIAAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10376 Human TUBA1B

GeneID: 22143 Mouse TUBA1B

GeneID: 500929 Rat TUBA1B

Gene Symbol TUBA1B
Gene Full Name tubulin, alpha 1b
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
Research Area Signaling Transduction antibody; Loading Control antibody
Calculated MW 50 kDa
PTM Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.
Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).
Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).

Images (2) Click the Picture to Zoom In

  • ARG22797 anti-alpha Tubulin antibody [YOL1/34] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG22797 anti-alpha Tubulin antibody [YOL1/34] (green), nuclei are counterstained with DAPI.

  • ARG22797 anti-alpha Tubulin antibody [YOL1/34] WB image

    Western blot: J774 cell lysate stained with anti-CD107b antibody at 1:500, 1:1000, 1:2000 dilution. ARG22797 anti-alpha Tubulin antibody [YOL1/34] is included as a loading control. Detection is with Goat anti-Rat IgG (Dylight).