ARG22797
anti-alpha Tubulin antibody [YOL1/34]
anti-alpha Tubulin antibody [YOL1/34] for ELISA,ICC/IF,IHC-Frozen sections,Radioimmunoassay,Western blot and Human,Mouse,Rat,Arabidopsis,Bird,D. melanogaster ,Fungi,Mammal,Yeast
Signaling Transduction antibody; Loading Control antibody
Overview
| Product Description | Rat Monoclonal antibody [YOL1/34] recognizes alpha Tubulin This antibody recognizes alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2. Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Arabi, Bird, Dm, Fungi, Mamm, Yeast |
| Tested Application | ELISA, ICC/IF, IHC-Fr, RIA, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YOL1/34 |
| Isotype | IgG2a |
| Target Name | alpha Tubulin |
| Antigen Species | Yeast |
| Immunogen | Yeast tubulin |
| Epitope | This antibody binds to an epitope between amino acids 414 and 422 of alpha tubulin (EEGEFTEAR). |
| Conjugation | Un-conjugated |
| Alternate Names | Tubulin K-alpha-1; Alpha-tubulin ubiquitous; Tubulin alpha-ubiquitous chain; Tubulin alpha-1B chain; K-ALPHA-1 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBA1B |
| Gene Full Name | tubulin, alpha 1b |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| Research Area | Signaling Transduction antibody; Loading Control antibody |
| Calculated MW | 50 kDa |
| PTM | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity). Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity). |
Images (2) Click the Picture to Zoom In
-
ARG22797 anti-alpha Tubulin antibody [YOL1/34] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG22797 anti-alpha Tubulin antibody [YOL1/34] (green), nuclei are counterstained with DAPI.
-
ARG22797 anti-alpha Tubulin antibody [YOL1/34] WB image
Western blot: J774 cell lysate stained with anti-CD107b antibody at 1:500, 1:1000, 1:2000 dilution. ARG22797 anti-alpha Tubulin antibody [YOL1/34] is included as a loading control. Detection is with Goat anti-Rat IgG (Dylight).
