ARG52219
anti-alpha II Spectrin antibody
anti-alpha II Spectrin antibody for ICC/IF,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha II Spectrin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | alpha II Spectrin |
| Antigen Species | Human |
| Immunogen | Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin |
| Conjugation | Un-conjugated |
| Alternate Names | Spectrin alpha chain, non-erythrocytic 1; SPTA2; Fodrin alpha chain; EIEE5; NEAS; Alpha-II spectrin; Spectrin, non-erythroid alpha subunit |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~240 k alpha II spectrin protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Neat serum |
| Buffer | Neat serum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPTAN1 |
| Gene Full Name | spectrin, alpha, non-erythrocytic 1 |
| Background | The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004). |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 285 kDa |
| PTM | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
Images (3) Click the Picture to Zoom In
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ARG52219 anti-alpha II Spectrin antibody WB image
Western blot: Mouse brain lysate stained with ARG52219 anti-alpha II Spectrin antibody showing specific immunolabeling of the ~240k alpha II spectrin protein.
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ARG52219 anti-alpha II Spectrin antibody ICC/IF image
Immunofluorescence: cultured Rat neurons and glia showing axonal and dendritic staining of ARG52219 anti-alpha II Spectrin antibody (green) revealing the submembraneous cytoskeleton and nestin by ARG52345 anti-Nestin antibody [4D11] (red).
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ARG52219 anti-alpha II Spectrin antibody WB image
Western blot: Mouse sciatic nerve and Mouse brain homogenate lysates stained with ARG52219 anti-alpha II Spectrin antibody.
A prominent band running at 240 kDa respresents the intact alpha II Spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha II Spectrin.
Customer's Feedback
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Review for anti-alpha II Spectrin antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
