ARG52218
anti-alpha II Spectrin antibody [3D7]
anti-alpha II Spectrin antibody [3D7] for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Bovine
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [3D7] recognizes alpha II Spectrin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3D7 |
| Isotype | IgG1 |
| Target Name | alpha II Spectrin |
| Antigen Species | Human |
| Immunogen | Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin |
| Conjugation | Un-conjugated |
| Alternate Names | Spectrin alpha chain, non-erythrocytic 1; SPTA2; Fodrin alpha chain; EIEE5; NEAS; Alpha-II spectrin; Spectrin, non-erythroid alpha subunit |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~240 k alpha II spectrin protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS and 10 mM Sodium azide |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPTAN1 |
| Gene Full Name | spectrin, alpha, non-erythrocytic 1 |
| Background | The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004). |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 285 kDa |
| PTM | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
Images (4) Click the Picture to Zoom In
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ARG52218 anti-alpha II Spectrin antibody [3D7] WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~240k alpha II spectrin stained with ARG52218 anti-alpha II Spectrin antibody [3D7].
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ARG52218 anti-alpha II Spectrin antibody [3D7] IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG52218 anti-alpha II Spectrin antibody [3D7] (green) at 1:2000 dilution, and costained with anti-GFAP antibody (red) at 1:5000 dilution.
Clone 3D7 stains the submembraneous cytoskeleton on neurons and strongly reveals the cell bodies and dendrites of Purkinje cells, while the GFAP antibody stains the processes of Bergmann glia and astrocytes.
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ARG52218 anti-alpha II Spectrin antibody [3D7] ICC/IF image
Immunofluorescence: cultured Rat neurons and glia stained with ARG52218 anti-alpha II Spectrin antibody [3D7] showing axonal and dendritic staining of alpha II spectrin (green) revealing the submembraneous cytoskeleton and DNA (blue).
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ARG52218 anti-alpha II Spectrin antibody [3D7] WB image
Western blot: Rat whole brain, Rat spinal cord, Mouse whole brain, Mouse spinal cord, NIH/3T3, HEK293, HeLa, SH-SY5Y and C6 cell lysates stained with ARG52218 anti-alpha II Spectrin antibody [3D7] (green).
