ARG65781
anti-Zika virus NS1 antibody [SQab1609]
anti-Zika virus NS1 antibody [SQab1609] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Publication9
Overview
| Product Description | Mouse Monoclonal antibody [SQab1609] recognizes Zika virus NS1 |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This Zika virus NS1 antibody specifically reacts to Zika virus NS1 protein and do not cross-react to DENV and CHIKV tested by ELISA. And a paper published by Bollweg BC et al, 2017 also validated that this antibody do not cross-react to other flaviviruses and chikungunya virus by IHC-P samples. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab1609 |
| Isotype | IgG1 |
| Target Name | Zika virus NS1 |
| Antigen Species | Virus |
| Immunogen | Zika virus NS1 |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | ELISA: The antibody can be used as capture antibody in sandwich ELISA for Zika virus NS1 detection in combination with ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) as detection antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal. |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Zika virus nonstructural protein 1 |
|---|---|
| Background | The mosquito-borne Zika virus (ZIKV) is prompting worldwide concern due to its connection to neurological disorders including microcephaly. Zika virus belongs to the flavivirus family which encompasses Dengue, yellow fever, West Nile and more different viruses. The connection of neurological disorders creates a need for further research into the ZIKV infection and therapeutic approaches. For more ZIKA virus antibody and ZIKA virus antibody Duo products, please refer to Choose the Best ZIKA Virus Antibodies |
| Highlight | Related Antibody Duos and Panels: ARG30277 Zika virus NS1 ELISA Duo Related products: Zika virus antibodies; Zika virus Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Choose the Best ZIKA Virus Antibodies Best Zika Virus antibodies cited by CDC & A*STAR Exploring Antiviral Immune Response |
Images (7) Click the Picture to Zoom In
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] ICC/IF image
Immunofluorescence: ARG65781 anti-Zika virus NS1 antibody [SQab1609] (1:400) were used for detecting ZIKV NS1.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] FACS image
Flow Cytometry: Zika virus NS1 expressing plasmid transfected (red) or non-transfected (blue) 293T cells stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:1000 dilution.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image
Western blot: 20 μg of 293T cell lysates transfected with 1) control plasmid or 2) Zika virus NS1 expression plasmid. The blots were stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:5000 dilution.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image
Wetern blot: 100 ng of 1) ZIKV NS1 (Purified from E.coli), 2) DENV1 NS1 (Purified from 293 cell), 3) DENV2 NS1 (Purified from 293 cell), 4) DENV3 NS1 (Purified from 293 cell), 5) DENV4 NS1 (Purified from 293 cell), and 6) CHIKV NS1 (Purified from 293 cell) stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:5000 dilution.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image
Western blot: 10 μg of Vero cells Infected with 1) Mock, 2) ZIKV, 3) DENV1, 4) DENV2, 5) DENV3 and 6) DENV 4. Cell lysates were stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:2000.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] & ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) ELISA image
ELISA: 293 cells expressed DENV1-4, CHIKV NS1 protein and E.coli expressed ZIKV NS1 protein detected by ARG65781 anti-Zika virus NS1 antibody [SQab1609] as capture antibody (5 µg/ml), and ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) (1 µg/ml) as detection antibody, followed by incubation with streptavidin-HRP.
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ARG65781 anti-Zika virus NS1 antibody [SQab1609] ELISA image
ELISA: ARG65781 anti-Zika virus NS1 antibody [SQab1609] (1:5000) were used for detecting ZIKV NS1. Each protein (1 µg/ml) were coated onto ELISA plate.
Specific References
Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon
WB / Zika virus
Differential proteomics of Zika virus (ZIKV) infection reveals molecular changes potentially involved in immune system evasion by a Brazilian strain of ZIKV
IHC-P / Zika virus
Decreased CSTB, RAGE, and Axl Receptor Are Associated with Zika Infection in the Human Placenta
IHC-P / Zika virus
The Prevalence of Adenoid Hypertrophy among Children with Zika Related Microcephaly.
IHC-P / Zika virus
Zika Virus Infects Human Placental Mast Cells and the HMC-1 Cell Line, and Triggers Degranulation, Cytokine Release and Ultrastructural Changes.
ICC/IF, IHC-P, FACS / Zika virus
Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil.
IHC-P / Zika virus
Monodelphis domestica as a fetal intra-cerebral inoculation model for Zika virus pathogenesis
IHC-Fr / Zika virus
Optimization of commercially available Zika virus antibodies for use in a laboratory-developed immunohistochemical assay
IHC-P / Zika virus
Placental Histopathology and Clinical Presentation of Severe Congenital Zika Syndrome in a Human Immunodeficiency Virus-Exposed Uninfected Infant.
IHC-P / Zika virus
