ARG56165

anti-ZO2 antibody

anti-ZO2 antibody for Western blot and Human,Mouse

nuts_pic
2
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes ZO2
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ZO2
Antigen Species Human
Immunogen Recombinant protein of Human ZO-2.
Conjugation Un-conjugated
Alternate Names X104; DFNA51; Tight junction protein ZO-2; C9DUPq21.11; Zonula occludens protein 2; ZO2; Zona occludens protein 2; PFIC4; DUP9q21.11; Tight junction protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21873 Mouse TJP2

GeneID: 9414 Human TJP2

Swiss-port # Q9UDY2 Human Tight junction protein ZO-2

Swiss-port # Q9Z0U1 Mouse Tight junction protein ZO-2

Gene Symbol TJP2
Gene Full Name tight junction protein 2
Background This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Function Plays a role in tight junctions and adherens junctions. [UniProt]
Highlight Related products:
Anti-Rabbit IgG secondary antibodies;
Related poster download:
Dual role of TGF beta in cancer promotion and suppression.pdf
Calculated MW 134 kDa

Images (1) Click the Picture to Zoom In

  • ARG56165 anti-ZO2 antibody WB image

    Western blot: HepG2 cell lysate stained with ARG56165 anti-ZO2 antibody.

Customer's Feedback

nuts_pic      Average

Review for anti-ZO2 antibody

Application:WB

Sample:3T3

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-ZO2 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.

WB / Mouse

Yang Chung-Chih et al.
Int J Cancer.,  (2018)

publication_link

 

hr_line