ARG67181

anti-VHA-A antibody

anti-VHA-A antibody for Western blot and Arabidopsis

Overview

Product Description Rabbit Polyclonal antibody recognizes VHA
Tested Reactivity Arabi
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VHA
Antigen Species Plant
Immunogen Synthetic peptide corresponding to N-terminus of arabidopsis thaliana VHA protein.
Conjugation Un-conjugated
Alternate Names V-type proton ATPase catalytic subunit A; V-ATPase subunit A; V-ATPase 69 kDa subunit; Vacuolar H(+)-ATPase subunit A; Vacuolar proton pump subunit alpha

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 68 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol VHA-A
Gene Full Name vacuolar ATP synthase subunit A
Background Encodes catalytic subunit A of the vacuolar ATP synthase. Mutants are devoid of vacuolar ATPase activity as subunit A is encoded only by this gene and show strong defects in male gametophyte development and in Golgi stack morphology.
Function Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. [UniProt] V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProt]
Cellular Localization Vacuole membrane. [UniProt]
Calculated MW 68 kDa

Images (1) Click the Picture to Zoom In

  • ARG67181 anti-VHA-A antibody WB image

    Western blot: Arabidopsis thaliana stained with ARG67181 anti-VHA-A antibody.