ARG10513
anti-VEGF antibody [VG-1]
anti-VEGF antibody [VG-1] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Dog,Rabbit
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Publication11
Overview
| Product Description | Mouse Monoclonal antibody [VG-1] recognizes VEGF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Rb |
| Species Does Not React With | Cow |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | Detects the 121, 165 and 189 VEGF isoforms in fixed specimens. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | VG-1 |
| Isotype | IgG1 |
| Target Name | VEGF |
| Immunogen | Recombinant VEGF 189 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Suggested blocking buffer: 3% milk blocking for 1 hr at RT (better than at 4°C, overnight). Recommend primary and secondary dilution buffer: TBST. IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 (recommended) or citrate buffer pH6.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Corpus luteum, VEGF165 recombinant protein, astrocytoma or hemoangiosarcoma. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VEGFA |
| Gene Full Name | Vascular endothelial growth factor A |
| Background | VEGF is an angiogenic growth factor and a prognostic indicator for cancer when detected in serum. VG-1 is useful for studies of VEGF and angiogenesis in human pathological material. |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [Uniprot] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 16 - 45 kDa |
Images (3) Click the Picture to Zoom In
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ARG10513 anti-VEGF antibody [VG-1] IHC-P image
Immunohistochemistry: Mouse formula stained with ARG10513 anti-VEGF antibody [VG-1].
From Chenjie Xia et al. J Orthop Surg Res. (2023), doi: 10.1186/s13018-023-03696-7, Fig. 7A.
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ARG10513 anti-VEGF antibody [VG-1] ICC/IF image
Immunofluorescence: Hamstring tenocytes treated with ARG10513 anti-VEGF antibody [VG-1].
From Cheng-Chang Lu et al. Bone Joint Res ., (2023) doi: doi.org/10.1302/2046-3758.121.BJR-2021-0434.R2, Fig. 6C.
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ARG10513 anti-VEGF antibody [VG-1] WB image
Western blot: Mouse gastrocnemius stained with ARG10513 anti-VEGF antibody [VG-1].
From Yong-Qing Do et al. Theranostics. (2020), doi: 10.7150/thno.39320, Fig. 2A.
Specific References
Effect of lyophilized exosomes derived from umbilical cord stem cells on chronic anterior cruciate ligament cell injury
ICC/IF / Human
Network pharmacology-based mechanism prediction and pharmacological validation of Bushenhuoxue formula attenuating postmenopausal osteoporosis in ovariectomized mice
IHC-P / Mouse
Anterior cruciate ligament remnant preservation attenuates apoptosis and enhances the regeneration of hamstring tendon graft
ICC/IF / Rabbit
Exploring the mechanism of Bushenhuoxue formula acting on postmenopausal osteoporosis via network pharmacology and experimental validation.
IHC-P / Mouse
Extracorporeal shock wave promotes activation of anterior cruciate ligament remnant cells and their paracrine regulation of bone marrow stromal cells' proliferation, migration, collagen synthesis, and differentiation.
ICC/IF / Human
Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.
WB / Human
Yougui pills exert osteoprotective effects on rabbit steroid-related osteonecrosis of the femoral head by activating β-catenin.
IHC-P / Rabbit
Long noncoding RNA CPS1-IT1 suppresses melanoma cell metastasis through inhibiting Cyr61 via competitively binding to BRG1.
WB / Human
Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.
WB / Mouse
An EGLN1 mutation may regulate hypoxic response in cyanotic congenital heart disease through the PHD2/HIF-1A pathway
WB / Human
Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling
IHC-P / Mouse
