ARG66387

anti-V5 tag antibody [SQab18114]

anti-V5 tag antibody [SQab18114] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab18114] recognizes V5 tag
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab18114
Isotype IgG
Target Name V5 tag
Antigen Species Others
Immunogen GKPIPNPLLGLDST (V5 epitope) conjugated to KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:2000 - 1:10000
ICC/IF1:2000 - 1:10000
IP1:50
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (6) Click the Picture to Zoom In

  • ARG66387 anti-V5 tag antibody [SQab18114] ICC/IF image

    Immunofluorescence: 293 cells transfected with N-terminal V5 tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Samples were stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:10000 dilution at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG66387 anti-V5 tag antibody [SQab18114] FACS image

    Flow Cytometry: 293 cells transfected with N-terminal (red) or C-terminal (green) V5 tagged gene and stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:10000 dilution in 1x PBS/1% BSA for 30 min at at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66387 anti-V5 tag antibody [SQab18114] WB image

    Western blot: 2 µg of 1) 293 cells transfected with N-terminal V5 tagged gene and stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:10000 dilution. 2) 293 cells transfected with C-terminal V5 tagged gene and stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:2000 dilution. 3) Mock 293 cells stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:2000 dilution.

  • ARG66387 anti-V5 tag antibody [SQab18114] IP image

    Immunoprecipitation: 0.1 mg of 293 whole cell lysate (transfected with N-terminal V5 tagged gene) was immunoprecipitated (1:50 dilution) and stained with ARG66387 anti-V5 tag antibody [SQab18114].

    Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with N-terminal V5 tagged gene)
    Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with N-terminal V5 tagged gene)
    Lane 3: 293 whole cell lysate (transfected with N-terminal V5 tagged gene), 4 µg (input)

  • ARG66387 anti-V5 tag antibody [SQab18114] ICC/IF image

    Immunofluorescence: 293 cells transfected with C-terminal V5 tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Samples were stained with ARG66387 anti-V5 tag antibody [SQab18114] at 1:10000 dilution at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG66387 anti-V5 tag antibody [SQab18114] IP image

    Immunoprecipitation: 0.1 mg of 293 whole cell lysate (transfected with C-terminal V5 tagged gene) was immunoprecipitated (1:50 dilution) and stained with ARG66387 anti-V5 tag antibody [SQab18114].

    Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with C-terminal V5 tagged gene)
    Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with C-terminal V5 tagged gene)
    Lane 3: 293 whole cell lysate (transfected with C-terminal V5 tagged gene), 4 µg (input)