ARG62608

anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)]

anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Goat,Guinea pig,Pig,Rabbit,Sheep,Zebrafish

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
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Overview

Product Description Mouse Monoclonal antibody [13C4 (I3C4)] recognizes UCHL1 / PGP9.5
Tested Reactivity Hu, Ms, Rat, Dog, Goat, Gpig, Pig, Rb, Sheep, Zfsh
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 13C4 (I3C4)
Isotype IgG
Target Name UCHL1 / PGP9.5
Antigen Species Human
Immunogen Human brain
Conjugation Un-conjugated
Alternate Names PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:30
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22223 Mouse UCHL1

GeneID: 29545 Rat UCHL1

GeneID: 396637 Pig UCHL1

Gene Symbol UCHL1
Gene Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Background The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW 25 kDa
PTM O-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] WB image

    Western blot: 30 µg of Mouse brain lysate stained with ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] at 1:1000 dilution.

  • ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] WB image

    Western blot: 30 µg of Rat brain lysate stained with ARG62608 anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)] at 1:2000 dilution.

Customer's Feedback

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Review for anti-UCHL1 / PGP9.5 antibody [13C4 (I3C4)]

Application:WB

Sample:1) A549, 2) H1299 cells

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Telocytes as a Novel Structural Component in the Muscle Layers of the Goat Rumen.

IHC-P / Goat

Liang Yu et al.
Cell Transplant.,  (2019)

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