ARG52461

anti-Tyrosine Hydroxylase antibody

anti-Tyrosine Hydroxylase antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Mammal

Cancer antibody; Metabolism antibody; Neuroscience antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Tyrosine Hydroxylase
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Tyrosine Hydroxylase
Antigen Species Rat
Immunogen SDS-denatured rat tyrosine hydroxylase purified from pheochromocytoma
Conjugation Un-conjugated
Alternate Names DYT14; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH; DYT5b; EC 1.14.16.2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
IHC-P1:500 - 1:1500
WB1:1000
Application Note Specific for the ~60k tyrosine hydroxylase protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21823 Mouse TH

GeneID: 25085 Rat TH

GeneID: 7054 Human TH

Gene Symbol TH
Gene Full Name tyrosine hydroxylase
Background Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
Highlight Related Antibody Duos and Panels:
ARG30212 Phospho Tyrosine Hydroxylase Antibody Panel (Total, pS31, pS40)
Related products:
Tyrosine Hydroxylase antibodies; Tyrosine Hydroxylase Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 59 kDa

Images (4) Click the Picture to Zoom In

  • ARG52461 anti-Tyrosine Hydroxylase antibody WB image

    Western blot: 30 µg of Mouse brain tissue lysate stained with ARG52461 anti-Tyrosine Hydroxylase antibody at 1:500 dilution.

  • ARG52461 anti-Tyrosine Hydroxylase antibody ICC/IF image

    Immunofluorescence: E17 Rat midbrain mixed neuronal cultures stained with ARG52461 anti-Tyrosine Hydroxylase antibody (green) and ARG52328 anti-MAP2 antibody (red).

  • ARG52461 anti-Tyrosine Hydroxylase antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse brain stained with ARG52461 anti-Tyrosine Hydroxylase antibody at 1:1000 dilution.

  • ARG52461 anti-TH antibody WB image

    Western Blot: 10 μg of rat caudate lysate showing specific immunolabeling of the ~60k TH protein stained with TH antibody (ARG52461).

Customer's Feedback

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Review for anti-Tyrosine Hydroxylase antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation

Imaging Cytometry / Human

Yu-Che Cheng et al.
Stem Cell Rev Rep.,  (2022)

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