anti-Type II Cytokeratins antibody [SQab1885]
anti-Type II Cytokeratins antibody [SQab1885] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human
Signaling Transduction antibody; Carcinoma Marker antibody
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1885] recognizes Type II Cytokeratins|
|Tested Application||FACS, IHC-P, WB|
|Target Name||Type II Cytokeratins|
|Immunogen||Synthetic peptide corresponding to aa. 300-400 of Human Type-II Cytokeratins.|
|Alternate Names||67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||keratin 1, type II|
|Background||The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]|
|Function||May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt]|
Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|Research Area||Signaling Transduction antibody; Carcinoma Marker antibody|
|Calculated MW||51 - 66 kDa|
|PTM||Undergoes deimination of some arginine residues (citrullination). [UniProt]|
Images (3) Click the Picture to Zoom In
ARG66337 anti-Type II Cytokeratins antibody [SQab1885] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] (red) at 1:50 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66337 anti-Type II Cytokeratins antibody [SQab1885] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
ARG66337 anti-Type II Cytokeratins antibody [SQab1885] WB image
Western blot: 10 µg of A549, SW480, HaCat and Caco-2 cell lysates stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] at 1:10000 dilution.