ARG64172
anti-TRAF2 antibody
anti-TRAF2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TRAF2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRAF2 |
| Antigen Species | Human |
| Immunogen | C-KMEAKNSYVRDD |
| Conjugation | Un-conjugated |
| Alternate Names | TRAP3; EC 6.3.2.-; E3 ubiquitin-protein ligase TRAF2; MGC:45012; TRAP; Tumor necrosis factor type 2 receptor-associated protein 3; TNF receptor-associated factor 2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| Calculated MW | 56 kDa |
| PTM | Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. |
Images (10) Click the Picture to Zoom In
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ARG64172 anti-TRAF2 antibody WB image
Western Blot: human ovary lysate (35 µg protein in RIPA buffer) stained with ARG64172 anti-TRAF2 antibody at 0.1 µg/ml dilution.
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ARG64172 anti-TRAF2 antibody WB image
Western Blot: 1). Mock transfection; 2) Human TRAF2 (RC208110) expressing plasmid transfected HEK293 cell lysate standed with ARG64172 anti-TRAF2 antibody
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ARG64172 anti-TRAF2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64172 anti-TRAF2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64172 anti-TRAF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64172 anti-TRAF2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64172 anti-TRAF2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64172 anti-TRAF2 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG64172 anti-TRAF2 antibody WB image
Western blot: 35 µg of HepG2 (A), Human ovary (B) and Jurkat (C) lysates (in RIPA buffer) stained with ARG64172 anti-TRAF2 antibody at 0.1 µg/ml (A), 0.5 µg/ml (B) and 0.1 µg/ml (C) dilutions and incubated at RT for 1 hour.
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ARG64172 anti-TRAF2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG64172 anti-TRAF2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64172 anti-TRAF2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG64172 anti-TRAF2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64172 anti-TRAF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64172 anti-TRAF2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64172 anti-TRAF2 antibody WB image
Western blot: 35 µg of Mouse testis lysate (in RIPA buffer) stained with ARG64172 anti-TRAF2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
