ARG53680
anti-TRADD antibody
anti-TRADD antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TRADD |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRADD |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from C-terminus of human TRADD. |
| Conjugation | Un-conjugated |
| Alternate Names | TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; Hs.89862; Tumor necrosis factor receptor type 1-associated DEATH domain protein |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Liver Carcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and <0.1% Sodium azide |
| Preservative | <0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15628 Human Tumor necrosis factor receptor type 1-associated DEATH domain protein |
|---|---|
| Gene Symbol | TRADD |
| Gene Full Name | TNFRSF1A-associated via death domain |
| Background | The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008] |
| Function | The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. [UniProt] |
| Cellular Localization | Cytoplasm |
| Highlight | Related products: TRADD antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 34 kDa |
Images (2) Click the Picture to Zoom In
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ARG53680 anti-TRADD antibody WB image
Western blot: 30 μg of HeLa cell 1) with no treatment, or 2) treat with 5 ng/ml TNF-a for 5 min. The blots were stained with ARG53680 anti-TRADD antibody at 1:500 dilution.
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ARG53680 anti-TRADD antibody IHC-P image
Immunohistochemistry: Human Liver Carcinoma stained with TRADD antibody (ARG53680)
