ARG65676
anti-TNFAIP1 antibody
anti-TNFAIP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TNFAIP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TNFAIP1 |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human TNFAIP1 (NP_066960.1). |
| Conjugation | Un-conjugated |
| Alternate Names | BTB/POZ domain-containing protein TNFAIP1; hBACURD2; B61; BTBD34; EDP1; Tumor necrosis factor, alpha-induced protein 1, endothelial; B12; Protein B12; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human colon cancer, Human thyroid cancer. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13829 Human BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation prote |
|---|---|
| Gene Symbol | TNFAIP1 |
| Gene Full Name | tumor necrosis factor, alpha-induced protein 1 (endothelial) |
| Background | This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008] |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 36 kDa |
| PTM | Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA. |
Images (4) Click the Picture to Zoom In
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ARG65676 anti-TNFAIP1 antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human colon cancer tissue stained with ARG65676 anti-TNFAIP1 antibody (left) at 1:25 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG65676 anti-TNFAIP1 antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human thyroid cancer tissue stained with ARG65676 anti-TNFAIP1 antibody (left) at 1:25 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
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ARG65676 anti-TNFAIP1 antibody WB image
WB analysis of HepG2, Huh7, A549 and H1299 cell lysate stained with ARG65676 anti-TNFAIP1 antibody.
Image reproduced under the Creative Commons license from Li Lihui et al. Signal Transduct Target Ther., (2020), Fig. 1c-e.
Doi: 10.1038/s41392-020-0140-z -
ARG65676 anti-TNFAIP1 antibody WB image
WB analysis of control, TNFAIP1 or BTBD9 sgRNA stable expressed A549 and H1299 cell lysate stained with ARG65676 anti-TNFAIP1 antibody.
Image reproduced under the Creative Commons license from Li Lihui et al. Signal Transduct Target Ther., (2020), Fig. 4a and 4b.
Doi: 10.1038/s41392-020-0140-z
Specific References
The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration.
WB / Human
