ARG43400
anti-TNF alpha antibody (Biotin)
anti-TNF alpha antibody (Biotin) for ELISA,Western blot and Rat
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes TNF alpha |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TNF alpha |
| Antigen Species | Rat |
| Immunogen | E. coli-derived recombinant Rat TNF alpha. |
| Conjugation | Biotin |
| Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Sandwich ELISA (Capture antibody - Detection antibody): ARG56624 - ARG43400 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNF |
| Gene Full Name | tumor necrosis factor |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted. C-domain 1: Secreted. C-domain 2: Secreted. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43400 anti-TNF alpha antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Rat TNF alpha protein stained with ARG43400 anti-TNF alpha antibody (Biotin), under reducing conditions.
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ARG43400 anti-TNF alpha antibody (Biotin) standard curve image
Sandwich ELISA: ARG43400 anti-TNF alpha antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56624 anti-TNF alpha antibody. Results of a typical standard run with optical density reading at 450 - 620 nm.
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ARG43400 anti-TNF alpha antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Rat TNF alpha protein stained with ARG43400 anti-TNF alpha antibody (Biotin), under non-reducing conditions.
