ARG10158
anti-TNF alpha antibody [2C8]
anti-TNF alpha antibody [2C8] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Publication7
Overview
| Product Description | Mouse Monoclonal antibody [2C8] recognizes TNF alpha |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, Neut, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2C8 |
| Isotype | IgG1 |
| Target Name | TNF alpha |
| Antigen Species | Human |
| Immunogen | Human recombinant tumor necrosis factor of alpha type |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Application Instructions
| Application Suggestion |
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| Application Note | Neutralizing: The clone [2C8] inhibit biological activity of TNF-alpha. The antibody concentration is depending on the test design from 10 ng/ml to 5 µg/ml. ELISA: The clone 2C8 can be used as a capture antibody in sandwich ELISA in combination with a tracer/detection antibody clone [F6C5] (Cat. No.: ARG10441). IHC-P: Antigen Retrieval: Boil tissue section in EDTA pH8.0 or pH9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.4) and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNF |
| Gene Full Name | tumor necrosis factor |
| Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt] |
| Highlight | Related products: TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 26 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. |
Images (3) Click the Picture to Zoom In
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ARG10158 anti-TNF alpha antibody [2C8] IHC-P image
Immunohistochemistry: Mouse Renal tissue stained with ARG10158 anti-TNF alpha antibody [2C8].
From Hanxu Zeng et al. Inflamm Res. (2020), doi: 10.1007/s00011-020-01411-4, Fig. 10a.
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ARG10158 anti-TNF alpha antibody [2C8] WB image
Western blot: Mouse Renal tissue stained with ARG10158 anti-TNF alpha antibody [2C8].
From Hanxu Zeng et al. Inflamm Res. (2020), doi: 10.1007/s00011-020-01411-4, Fig. 10a.
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ARG10158 anti-TNF alpha antibody [2C8] IHC-Fr image
Immunohistochemistry: Frozen section of human joint from a patient with rheumatoid arthritis stained with ARG10158 anti-TNF alpha antibody [2C8].
Specific References
Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses
WB / Mouse
Exosomal miR-7002-5p derived from highglucose-induced macrophages suppresses autophagy in tubular epithelial cells by targeting Atg9b
IHC-P / Mouse
Paeoniflorin ameliorates diabetic liver injury by targeting the TXNIP-mediated NLRP3 inflammasome in db/db mice
IHC-P, WB / Mouse
Facile open-well immunofluorescence enhancement with coplanar-electrodes-enabled optoelectrokinetics and magnetic particles
ELISA (Capture) / Human
Bruton's tyrosine kinase regulates macrophage-induced inflammation in the diabetic kidney via NLRP3 inflammasome activation.
WB / Mouse
Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.
IHC-P / Human
TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.
WB, IHC-P / Mouse
