ARG10002
anti-TGF beta antibody [TB21]
anti-TGF beta antibody [TB21] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse,Rat,Rabbit,Sheep
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody
Publication8
Overview
| Product Description | Mouse Monoclonal antibody [TB21] recognizes TGF beta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Rb, Sheep |
| Predict Reactivity | Cow, Mink, Pig |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, Neut, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TB21 |
| Isotype | IgG1, kappa |
| Target Name | TGF beta |
| Antigen Species | Human |
| Immunogen | TGF-β from human platelets |
| Conjugation | Un-conjugated |
| Alternate Names | TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1 |
Application Instructions
| Application Suggestion |
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| Application Note | Neutralizing: This antibody neutralizes TGF-β activity in vitro and in vivo. In an inhibition assay of CCL/64 cell growth and in a NRK-49F colony forming assay, the antibody neutralized TGF-β bioactivities. The effect of micro-injection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was also able to neutralize the bioactivity of TGF-β in vivo. IHC: This antibody has been used to demonstrate TGF-β in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes, it is possible to locate a single cell within the ovarian stroma, making it useful in locating very fine capillary networks within tissue. Western Blot: This antibody when used at concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.0) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TGFB1 |
| Gene Full Name | transforming growth factor, beta 1 |
| Background | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009] |
| Function | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 44 kDa |
| PTM | Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. |
Images (5) Click the Picture to Zoom In
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ARG10002 anti-TGF beta antibody [TB21] IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG10002 anti-TGF beta antibody [TB21] at 1:200 dilution.
From Chi-Chih Hung et al. Cancers (Basel). (2020), doi: 10.3390/cancers12123561, Fig. 6.
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ARG10002 anti-TGF beta antibody [TB21] WB image
Western blot: Mouse kidney stained with ARG10002 anti-TGF beta antibody [TB21] at 1:500 dilution.
From Chi-Chih Hung et al. Cancers (Basel). (2020), doi: 10.3390/cancers12123561, Fig. 7A, B.
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ARG10002 anti-TGF beta antibody [TB21] IHC-Fr image
Immunohistochemistry: Mouse tibiae stained with ARG10002 anti-TGF beta antibody [TB21] at 1:200 dilution.
From Chenjie Xia et al. Cell Prolif. (2020), doi: 10.1111/cpr.12904, Fig. 4A.
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ARG10002 anti-TGF beta antibody [TB21] WB image
Western blot: 30 µg of Rat spleen and Rat heart lysates stained with ARG10002 anti-TGF beta antibody [TB21] at 1:1000 dilution.
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ARG10002 anti-TGF beta antibody [TB21] WB image
Western Blot: 1. Platelet-derived TGF-b (12 μg/lane), 2. Platelet-derived TGF-b (6 μg/lane), 3. Platelet-derived TGF-b (3 μg/lane), 4. Platelet-derived TGF-b (1 μg/lane), stained with anti-TGF beta antibody [TB21] (ARG10002) at 2µg/mL
The bottom band is the 12.5KD TGF-b monomer, the mid-band is the 25KD TGF-b dimer and the up band is TGF-b with signal peptide. Lane 1 is molecular weight markers.
Customer's Feedback
Good
Review for anti-TGF beta antibody [TB21]
Application：WB
Sample：Rat spleen and Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-TGF beta antibody [TB21]
Application：WB
Sample：Mouse spleen
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Effect of lyophilized exosomes derived from umbilical cord stem cells on chronic anterior cruciate ligament cell injury
ICC/IF / Human
Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses
WB / Mouse
Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells
WB / Human
Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.
WB / Mouse
Combination of optimized tissue engineering bone implantation with heel-strike like mechanical loading to repair segmental bone defect in New Zealand rabbits
WB / Rabbit
TGF-β/Smad2 signalling regulates enchondral bone formation of Gli1 + periosteal cells during fracture healing.
IHC-Fr / Mouse
Lung Cancer Cell-Derived Secretome Mediates Paraneoplastic Inflammation and Fibrosis in Kidney in Mice.
WB, IHC-P / Mouse
Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats.
WB / Rat
