ARG56078
anti-TGF beta antibody [1D11.16.8]
anti-TGF beta antibody [1D11.16.8] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse,Rat,Cow,Dog,Hamster,Monkey
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [1D11.16.8] recognizes TGF beta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Dog, Hm, Mk |
| Tested Application | ELISA, ICC/IF, IHC-P, Neut, WB |
| Specificity | This mAb recognizes TGF beta 1, 2 and 3. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1D11.16.8 |
| Isotype | IgG1, kappa |
| Target Name | TGF beta |
| Antigen Species | Bovine |
| Immunogen | Bovine TGF-beta2 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | TGF-beta2; Cetermin; LDS4; Polyergin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Transforming growth factor beta-2; LAP; TGF-beta-2; BSC-1 cell growth inhibitor |
Application Instructions
| Application Suggestion |
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| Application Note | WB: It is recommended detct TGF beta under non-reducing conditions. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TGFB2 |
| Gene Full Name | transforming growth factor, beta 2 |
| Background | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
| Function | TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. [UniProt] |
| Cellular Localization | Cytoplasm and extracellular (secreted) |
| Calculated MW | 48 kDa |
| PTM | The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. |
Images (1) Click the Picture to Zoom In
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ARG56078 anti-TGF beta antibody [1D11.16.8] neutralization image
Neutralization assay: Primary microglial cultures neutralizing with ARG56078 anti-TGF beta antibody [1D11.16.8] at 0.2 μg/mL dilution.
mRNA expression of TNF-α and IL-10 in microglia cultured alone, cocultured with astrocyte by transwell and cultured by ACM in the presence or absence of the anti-TGF-β antibody.From Jinqiang Zhang et al. Front Cell Neurosci (2020), doi: 10.3389/fncel.2020.00195, Fig.8 D and E.
Specific References
Mouse Astrocytes Promote Microglial Ramification by Releasing TGF-β and Forming Glial Fibers.
Neutralizing / Mouse
