ARG59950

anti-TARS / ThrRS antibody

anti-TARS / ThrRS antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TARS / ThrRS
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TARS / ThrRS
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-230 of Human TARS (NP_689508.3).
Conjugation Un-conjugated
Alternate Names Threonyl-tRNA synthetase; Threonine--tRNA ligase, cytoplasmic; ThrRS; EC 6.1.1.3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NCI-H460
Observed Size 83 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 110960 Mouse TARS

GeneID: 294810 Rat TARS

GeneID: 6897 Human TARS

Gene Symbol TARS
Gene Full Name threonyl-tRNA synthetase
Background Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [provided by RefSeq, Jul 2008]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 83 kDa
PTM ISGylated. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG59950 anti-TARS / ThrRS antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG59950 anti-TARS / ThrRS antibody.

  • ARG59950 anti-TARS / ThrRS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat heart stained with ARG59950 anti-TARS / ThrRS antibody at 1:100 dilution.

  • ARG59950 anti-TARS / ThrRS antibody WB image

    Western blot: 25 µg of NCI-H460 cell lysate stained with ARG59950 anti-TARS / ThrRS antibody at 1:1000 dilution.

  • ARG59950 anti-TARS / ThrRS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG59950 anti-TARS / ThrRS antibody at 1:100 dilution.