ARG52439
anti-Syntaxin antibody [SP8]
anti-Syntaxin antibody [SP8] for Immunohistochemistry,Western blot and Human,Mouse,Rat,Hamster,Pig
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [SP8] recognizes Syntaxin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm, Pig |
| Tested Application | IHC, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SP8 |
| Isotype | IgG1 |
| Target Name | Syntaxin |
| Antigen Species | Human |
| Immunogen | Synaptic immunoprecipitate (crude) from human brain |
| Conjugation | Un-conjugated |
| Alternate Names | HPC-1; STX1; Syntaxin-1A; SYN1A; Neuron-specific antigen HPC-1; P35-1 |
Application Instructions
| Application Suggestion |
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| Application Note | The antibody is specific for the ~ 36 kDa syntaxin protein in Western blots of Rat brain lysate. The antibody has also been used for immunohistochemistry on formalin fixed, vibRatome sections. Does not work on paraffin sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STX1A |
| Gene Full Name | syntaxin 1A (brain) |
| Background | Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010). |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. |
Images (1) Click the Picture to Zoom In
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ARG52439 anti-Syntaxin antibody [SP8] WB image
Western Blot: rat cortex lysate showing specific immunolabeling of the ~ 36k syntaxin 1A protein stained with Syntaxin antibody [SP8] (ARG52439).
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Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µl
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
