ARG41421
anti-Serum amyloid P component antibody
anti-Serum amyloid P component antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Serum amyloid P component |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Serum amyloid P component |
| Antigen Species | Rat |
| Immunogen | Recombinant protein corresponding to Q21-S228 of Rat Serum amyloid P component. |
| Conjugation | Un-conjugated |
| Alternate Names | 9.5S alpha-1-glycoprotein; HEL-S-92n; PTX2; Serum amyloid P-component; 1-203; SAP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 28 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | APCS |
| Gene Full Name | amyloid P component, serum |
| Background | The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008] |
| Function | Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41421 anti-Serum amyloid P component antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41421 anti-Serum amyloid P component antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41421 anti-Serum amyloid P component antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat liver lysates stained with ARG41421 anti-Serum amyloid P component antibody at 0.5 µg/ml dilution, overnight at 4°C.
