Overview

Product Description	Rabbit Polyclonal antibody recognizes SUMO4
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, IP, WB
Specificity	This antibody might also reacts to SUMO2 and SUMO3 based on high sequence homology.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	SUMO4
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human SUMO4
Conjugation	Un-conjugated
Alternate Names	SUMO-4; IDDM5; SMT3H4; dJ281H8.4; Small ubiquitin-related modifier 4; Small ubiquitin-like protein 4

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	293T
Observed Size	~ 16 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 387082 Human SUMO4 Swiss-port # Q6EEV6 Human Small ubiquitin-related modifier 4
Gene Symbol	SUMO4
Gene Full Name	small ubiquitin-like modifier 4
Background	This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq, Jul 2008]
Function	Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. [UniProt]
Calculated MW	11 kDa
PTM	In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. [UniProt]

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