ARG42951
anti-SSR3 antibody
anti-SSR3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SSR3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SSR3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 8-23 of Human SSR3. (KQQSEEDLLLQDFSRN) |
| Conjugation | Un-conjugated |
| Alternate Names | TRAPG; Translocon-associated protein subunit gamma; Signal sequence receptor subunit gamma; TRAP-gamma; SSR-gamma |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 21 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SSR3 |
| Gene Full Name | signal sequence receptor, gamma (translocon-associated protein gamma) |
| Background | The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR is comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. The first two are glycosylated subunits and the latter two are non-glycosylated subunits. This gene encodes the gamma subunit, which is predicted to span the membrane four times. [provided by RefSeq, Aug 2010] |
| Function | TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 21 kDa |
Images (9) Click the Picture to Zoom In
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ARG42951 anti-SSR3 antibody ICC image
Immunocytochemistry: HeLa cells were blocked with 10% goat serum and then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostatic cancer tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody WB image
Western blot: 50 µg of Rat liver, 50 µg of Rat spleen, 40 µg of A431, 40 µg of HeLa, 40 µg of U87 and 40 µg of SMMC-7721 whole cell lysates stained with ARG42951 anti-SSR3 antibody at 0.5 µg/ml dilution.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Mouse brain tissue stained with ARG42951 anti-SSR3 antibody at 2 μg/mL dilution.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
