ARG55040

anti-SQSTM1 / p62 antibody

anti-SQSTM1 / p62 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes SQSTM1 / p62
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SQSTM1 / p62
Antigen Species Human
Immunogen Recombinant protein of Human SQSTM1 (NP_001135770.1)
Conjugation Un-conjugated
Alternate Names FTDALS3; p60; p62; EBI3-associated protein of 60 kDa; p62B; Sequestosome-1; Ubiquitin-binding protein p62; PDB3; OSIL; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBIAP; A170; ZIP3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 113894 Rat SQSTM1

GeneID: 18412 Mouse SQSTM1

GeneID: 8878 Human SQSTM1

Gene Symbol SQSTM1
Gene Full Name sequestosome 1
Background SQSTM1 / p62 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Function SQSTM1 / p62: Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Promotes relocalization of 'Lys-63'-linked ubiquitinated TMEM173/STING to autophagosomes (PubMed:29496741). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed:20452972, PubMed:28380357). [UniProt]
Highlight Related Antibody Duos and Panels:
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
Calculated MW 48 kDa
PTM Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2 (PubMed:25040165).
Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery (PubMed:27368102).

Images (6) Click the Picture to Zoom In

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:MCF7

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa and Rat

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa and A549

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy

WB / Human, Mouse

Qian Zhang et al.
Food Funct.,  (2023)

publication_link

 

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Alcohol induces hepatocytes necroptosis through the LC3/RIPK1/RIPK3 pathway

WB / Mouse

Yuelin Chen et al.
Food Chem Toxicol.,  (2023)

publication_link

 

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Poloxamer 407 and Hyaluronic Acid Thermosensitive Hydrogel-Encapsulated Ginsenoside Rg3 to Promote Skin Wound Healing

WB / Mouse

Xiaojuan Peng et al.
Front Bioeng Biotechnol.,  (2022)

publication_link

 

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

WB, IHC-P, ICC/IF / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

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Curcumin and tetrahydrocurcumin induce cell death in Ara-C-resistant acute myeloid leukemia.

WB / Human

Tseng Yu-Hsin et al.
Phytother Res.,  (2019)

publication_link

 

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