ARG54937
anti-SOD2 antibody
anti-SOD2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Publication5
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SOD2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SOD2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human SOD2 (NP_000627.2) |
| Conjugation | Un-conjugated |
| Alternate Names | MNSOD; Superoxide dismutase [Mn], mitochondrial; IPOB; EC 1.15.1.1; MVCD6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SOD2 |
| Gene Full Name | superoxide dismutase 2, mitochondrial |
| Background | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30274 SOD1 and SOD2 Antibody Duo Related products: SOD2 antibodies; SOD2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 25 kDa |
| PTM | Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity). |
Images (4) Click the Picture to Zoom In
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ARG54937 anti-SOD2 antibody WB image
Western blot: Mouse hippocampal stained with ARG54937 anti-SOD2 antibody.
From Ji-Ren An et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1136070, Fig. 7F.
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ARG54937 anti-SOD2 antibody WB image
Western blot: 30 µg of HeLa, HepG2, MDAMB231, HEK293T, Mouse heart, Mouse liver, and Rat heart lysates stained with ARG54937 anti-SOD2 antibody at 1:500 dilution.
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ARG54937 anti-SOD2 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54937 anti-SOD2 antibody (orange) at 1:10 dilution.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG54937 anti-SOD2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oophoroma stained with ARG54937 anti-SOD2 antibody at 1:100 dilution (400x lens).
Customer's Feedback
Excellent
Review for anti-SOD2 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:10
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-SOD2 antibody
Application：WB
Sample：PC3 cell line
Sample Loading Amount：20µg
Primary Antibody Dilution Factor：1:1,000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress
WB / Mouse
Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice
WB / Mouse
Dietary Fe 3 O 4 Nanozymes Prevent the Injury of Neurons and Blood-Brain Barrier Integrity from Cerebral Ischemic Stroke.
WB / Mouse
The antiepileptic drug levetiracetam promotes neuroblast differentiation and expression of superoxide dismutase in the mouse hippocampal dentate gyrus via PI3K/Akt signalling.
WB / Mouse, Rat
Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the D-Galactose-Induced Aging Mice via Its Antioxidant Effects.
WB, IHC-Fr / Mouse
