ARG56476
anti-SOAT1 antibody
anti-SOAT1 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SOAT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SOAT1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human SOAT 1. |
| Conjugation | Un-conjugated |
| Alternate Names | STAT; ACACT; Cholesterol acyltransferase 1; Sterol O-acyltransferase 1; ACAT-1; ACAT1; ACAT; SOAT; Acyl-coenzyme A:cholesterol acyltransferase 1; EC 2.3.1.26 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | TBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.1% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SOAT1 |
| Gene Full Name | sterol O-acyltransferase 1 |
| Background | The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011] |
| Function | Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cholesterol, the weakness of anaplastic large cell lymphoma (ALCL) |
| Calculated MW | 65 kDa |
Images (4) Click the Picture to Zoom In
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ARG56476 anti-SOAT1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human adrenal tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Tissue section was stained with ARG56476 anti-SOAT1 antibody at 1:200 dilution.
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ARG56476 anti-SOAT1 antibody WB image
Western blot: 30 µg of Rat liver and HepG2 cell lysates stained with ARG56476 anti-SOAT1 antibody.
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ARG56476 anti-SOAT1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells stained with ARG56476 anti-SOAT1 antibody (green) at 1:200 dilution.
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ARG56476 anti-SOAT1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed Mouse Macrophages stained with ARG56476 anti-SOAT1 antibody (red).
Specific References
Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses.
WB / Human
