ARG63411

anti-SNX5 antibody

anti-SNX5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody
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Overview

Product Description

Goat Polyclonal antibody recognizes SNX5

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity Both variants represent identical protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SNX5
Antigen Species Human
Immunogen SLLQSCIDLFKNN
Conjugation Un-conjugated
Alternate Names Sorting nexin-5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 6 µg/ml
WB1 - 3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27131 Human SNX5

GeneID: 296199 Rat SNX5

GeneID: 69178 Mouse SNX5

Background This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein binds to fanconi anemia complementation group A protein, but its function is unknown. This gene results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 47 kDa

Images (2) Click the Picture to Zoom In

  • ARG63411 anti-SNX5 antibody WB image

    Western Blot: Jurkat lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63411 anti-SNX5 antibody at 1 µg/ml dilution.

  • ARG63411 anti-SNX5 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63411 anti-SNX5 antibody at 4 µg/ml dilution followed by HRP-staining.

Customer's Feedback

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Review for anti-SNX5 antibody

Application:WB

Sample:1) Mouse kidney, 2) Rat kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-SNX5 antibody

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC