ARG56313
anti-SF3B2 antibody
anti-SF3B2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SF3B2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SF3B2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human SF3B2 |
| Conjugation | Un-conjugated |
| Alternate Names | Cus1; Spliceosome-associated protein 145; SF3b1; SAP145; Splicing factor 3B subunit 2; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SF3b145; SF3b150; SAP 145; SF3B145 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A375 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SF3B2 |
| Gene Full Name | splicing factor 3b, subunit 2, 145kDa |
| Background | This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008] |
| Function | Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. [UniProt] |
| Calculated MW | 100 kDa |
| PTM | Methylation at Arg-508 by PRMT9 is required for the interaction with SMN1. |
Images (3) Click the Picture to Zoom In
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ARG56313 anti-SF3B2 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG56313 anti-SF3B2 antibody.
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ARG56313 anti-SF3B2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver stained with ARG56313 anti-SF3B2 antibody at 1:100 dilution.
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ARG56313 anti-SF3B2 antibody WB image
Western blot: A375 cell lysate stained with ARG56313 anti-SF3B2 antibody.
