ARG11060
anti-SCGN / Secretagogin antibody
anti-SCGN / Secretagogin antibody for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow
Overview
| Product Description | Chicken Polyclonal antibody recognizes SCGN / Secretagogin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow |
| Tested Application | IHC-Fr, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | SCGN / Secretagogin |
| Antigen Species | Human |
| Immunogen | Full-length recombinant Human Secretagogin protein. |
| Conjugation | Un-conjugated |
| Alternate Names | SEGN; DJ501N12.8; setagin; SECRET; CALBL; Secretagogin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 27 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5mM Sodium azide and 50% Glycerol. |
| Preservative | 5mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SCGN |
| Gene Full Name | secretagogin, EF-hand calcium binding protein |
| Background | The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008] |
| Calculated MW | 32 kDa |
Images (2) Click the Picture to Zoom In
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ARG11060 anti-SCGN / Secretagogin antibody IHC-Fr image
Immunohistochemistry: Mouse brain olfactory bulb section stained with ARG11060 anti-SCGN / Secretagogin antibody (red) at 1:1000 dilution, and costained with mouse mAb to calretinin (green) at 1:500 dilution. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.
Secretagogin is highly expressed in the mitral and granular cell layers, while calretinin is in the glomerular layer. Cells that express secretagogin or calretinin only are red or green respectively. A number of cells containing both proteins appears orange-yellow. Mouse select image at left for larger view.
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ARG11060 anti-SCGN / Secretagogin antibody WB image
Western blot: Mouse olfactory bulb, Rat cerebellum and Cow cerebellum lysates stained with ARG11060 anti-SCGN / Secretagogin antibody at 1:1000 dilution.
