ARG66735

anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177]

anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] for ELISA,ICC/IF,Western blot and Virus

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Overview

Product Description Recombinant Human Monoclonal antibody [SQab20177] recognizes SARS-CoV / SARS-CoV-2 nucleocapsid protein
Tested Reactivity Virus
Tested Application ELISA, ICC/IF, WB
Specificity This antibody reacts to SARS-CoV nucleocapsid protein and SARS-CoV-2 nucleocapsid protein, but not reacts to MERS-CoV nucleocapsid protein.
Host Human
Clonality Monoclonal
Clone SQab20177
Isotype IgG1
Target Name SARS-CoV / SARS-CoV-2 nucleocapsid protein
Antigen Species Virus
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ICC/IF1:200 - 1:1000
WB1:500 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from antibody expressing HEK293 cell culture medium.
Purity > 98% (SDS-PAGE)
Buffer PBS (pH 7.4) and 0.03% Proclin 300
Preservative 0.03% Proclin 300
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Human IgG secondary antibodies;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
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Images (3) Click the Picture to Zoom In

  • ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] ICC/IF image

    Immunofluorescence: Mock transfected, SARS-CoV NP, SARS-CoV-2 NP, MERS-CoV NP, HCoV-NL63 NP expressing plasmid transfected 293T cells were stained with ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] at 1:500 dilution.

  • ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] WB image

    Western blot: HEK293 cells untransfected (right) or transfected with SARS-CoV-2 VLP (left). The blots were stained with ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] at 1:1000 dilution.

  • ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177] ELISA image

    ELISA: The plate was coated with SARS-CoV-2, SARS-CoV, MERS-CoV nucleocapsid protein or BSA control. Proteins were stained with serially diluted ARG66735 anti-SARS-CoV / SARS-CoV-2 nucleocapsid protein antibody [SQab20177].

Specific References

Rapid magneto-enzyme-linked immunosorbent assay for ultrasensitive protein detection

ELISA / Virus

Kavya L Singampalli  et al.
Anal Chim Acta,  (2022)

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Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.

Others / Virus

Jiran Li et al.
ACS Sens.,  (2021)

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